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Literature summary for 2.2.1.6 extracted from

  • Le, D.T.; Choi, J.
    FAD-independent and herbicide-resistant mutants of tobacco acetohydroxy acid synthase (2005), Bull. Korean Chem. Soc., 26, 916-920.
No PubMed abstract available
Search for more literature for 2.2.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant GST-tagged enzymes in Escherichia coli strain BL21(DE3) Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
R372S/F373P/D374V site-directed mutagenesis, mutation of the conserved motif 372RFDDR376 results in abolished FAD binding and highly reduced activity Nicotiana tabacum
R372S/F373P/D374V/D375E site-directed mutagenesis, mutation of the conserved motif 372RFDDR376 results in abolished FAD binding and highly reduced activity Nicotiana tabacum
R372S/F373P/D374V/D375E/R376Y site-directed mutagenesis, mutation of the conserved motif 372RFDDR376 results in abolished FAD binding and highly reduced activity, the mutant is resistant to herbocides Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
Cadre an imidazole herbicide, no inhibition of mutant R372S/F373P/D374V/D375E/R376Y Nicotiana tabacum
Londax a sulfonylurea herbicide, no inhibition of mutant R372S/F373P/D374V/D375E/R376Y Nicotiana tabacum
[1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide a triazolopyrimidine derivative herbicide, no inhibition of mutant R372S/F373P/D374V/D375E/R376Y Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information cofactor affinities of wild-type and mutant enzymes, overview Nicotiana tabacum
0.0117
-
 pyruvate wild-type enzyme Nicotiana tabacum
0.0289
-
 pyruvate mutant R372S/F373P/D374V/D375E Nicotiana tabacum
0.0757
-
 pyruvate mutant R372S/F373P/D374V/D375E/R376Y Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nicotiana tabacum the enzyme can act in anabolic or in catabolic function, the first enzyme contains the conserved motif 372RFDDR376, while the latter does not, the conserved motif 372RFDDR376 is a possible determinant of the FAD-dependent and herbicide-resistant properties of tobacco, overview ?
-
 ?
pyruvate Nicotiana tabacum the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine 2-acetolactate + CO2
-
 r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli strain BL21(DE3) by two steps of glutathione affinity chromatography to homogeneity Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme can act in anabolic or in catabolic function, the first enzyme contains the conserved motif 372RFDDR376, while the latter does not, the conserved motif 372RFDDR376 is a possible determinant of the FAD-dependent and herbicide-resistant properties of tobacco, overview Nicotiana tabacum ?
-
 ?
pyruvate
-
 Nicotiana tabacum 2-acetolactate + CO2
-
 r
pyruvate the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine Nicotiana tabacum 2-acetolactate + CO2
-
 r

Synonyms

Synonyms Comment Organism
acetohydroxy acid synthase
-
 Nicotiana tabacum
AHAS
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
FAD required for the enzyme in anabolic function, but not in catabolic function, the conserved motif 372RFDDR376 is a possible determinant of the FAD-dependent and herbicide-resistant properties of tobacco, overview Nicotiana tabacum
thiamine diphosphate
-
 Nicotiana tabacum