Protein Variants | Comment | Organism |
---|---|---|
M250A | large decrease in activity, increase in Km-value | Escherichia coli |
R276K | large decrease in activity, increase in Km-value | Escherichia coli |
W464L | decrease in activity, increase in Km-value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
pyruvate | wild-type, pH 7.6, 37°C | Escherichia coli | |
6 | - |
pyruvate | wild-type with His-tag, pH 7.6, 37°C | Escherichia coli | |
11.2 | - |
pyruvate | mutant W464L with His-tag, pH 7.6, 37°C | Escherichia coli | |
36 | - |
pyruvate | mutant M250A with His-tag, pH 7.6, 37°C | Escherichia coli | |
38 | - |
pyruvate | mutant R276K with His-tag, pH 7.6, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 pyruvate = 2-acetolactate + CO2 | specificity of carboligation and product liberation may be cumulative if the former is not completely committed | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
mutant M250A with His-tag, pH 7.6, 37°C | Escherichia coli |
2.8 | - |
mutant R276K with His-tag, pH 7.6, 37°C | Escherichia coli |
11.9 | - |
mutant W464L with His-tag, pH 7.6, 37°C | Escherichia coli |
17 | - |
wild-type with His-tag, pH 7.6, 37°C | Escherichia coli |
20 | - |
wild-type, pH 7.6, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.03 | - |
pyruvate | mutant M250A with His-tag, pH 7.6, 37°C | Escherichia coli | |
3.27 | - |
pyruvate | mutant R276K with His-tag, pH 7.6, 37°C | Escherichia coli | |
13.9 | - |
pyruvate | mutant W464L with His-tag, pH 7.6, 37°C | Escherichia coli | |
19.8 | - |
pyruvate | wild-type with His-tag, pH 7.6, 37°C | Escherichia coli | |
23.3 | - |
pyruvate | wild-type, pH 7.6, 37°C | Escherichia coli |