Protein Variants | Comment | Organism |
---|---|---|
D428E | 8% activity compared to wild-type | Escherichia coli |
D428N | 8% activity compared to wild-type | Escherichia coli |
E47A | 8% activity compared to wild-type | Escherichia coli |
E47Q | 10% activity compared to wild-type | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfometuron methyl | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
pyruvate | wild-type, 37°C, pH 7.6 | Escherichia coli | |
7 | - |
pyruvate | mutant D428N, 37°C, pH 7.6 | Escherichia coli | |
7.3 | - |
pyruvate | mutant E47Q, 37°C, pH 7.6 | Escherichia coli | |
7.7 | - |
pyruvate | mutant E47A, 37°C, pH 7.6 | Escherichia coli | |
36 | - |
pyruvate | mutant D428E, 37°C, pH 7.6 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | mutant D428N, Km-value 19 mM, mutant D428E, Km-value 35 mM | Escherichia coli | |
Mg2+ | wild-type, Km-value 0.01 mM, mutant D428N, Km-value 0.21 mM, mutant D428E, Km-value 0.36 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isozyme AHAS II | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 pyruvate = 2-acetolactate + CO2 | structural model of enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
mutant E47A, 37°C, pH 7.6 | Escherichia coli |
2 | - |
mutant E47Q, 37°C, pH 7.6 | Escherichia coli |
20 | - |
wild-type, 37°C, pH 7.6 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
sulfometuron methyl | mutant E47Q, 37°C, pH 7.6 | Escherichia coli | |
0.0006 | - |
sulfometuron methyl | mutant E47A, 37°C, pH 7.6 | Escherichia coli | |
0.0008 | - |
sulfometuron methyl | wild-type, 37°C, pH 7.6 | Escherichia coli | |
0.0032 | - |
sulfometuron methyl | mutant D428N, 37°C, pH 7.6 | Escherichia coli | |
0.006 | - |
sulfometuron methyl | mutant D428E, 37°C, pH 7.6 | Escherichia coli |