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Literature summary for 2.2.1.6 extracted from

  • Bar-Ilan, A.; Balan, V.; Tittmann, K.; Golbik, R.; Vyazmensky, M.; Hubner, G.; Barak, Z.; Chipman, D.M.
    Binding and activation of thiamin diphosphate in acetohydroxyacid synthase (2001), Biochemistry, 40, 11946-11954.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D428E 8% activity compared to wild-type Escherichia coli
D428N 8% activity compared to wild-type Escherichia coli
E47A 8% activity compared to wild-type Escherichia coli
E47Q 10% activity compared to wild-type Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
sulfometuron methyl
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
pyruvate wild-type, 37°C, pH 7.6 Escherichia coli
7
-
pyruvate mutant D428N, 37°C, pH 7.6 Escherichia coli
7.3
-
pyruvate mutant E47Q, 37°C, pH 7.6 Escherichia coli
7.7
-
pyruvate mutant E47A, 37°C, pH 7.6 Escherichia coli
36
-
pyruvate mutant D428E, 37°C, pH 7.6 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ mutant D428N, Km-value 19 mM, mutant D428E, Km-value 35 mM Escherichia coli
Mg2+ wild-type, Km-value 0.01 mM, mutant D428N, Km-value 0.21 mM, mutant D428E, Km-value 0.36 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
isozyme AHAS II
-

Reaction

Reaction Comment Organism Reaction ID
2 pyruvate = 2-acetolactate + CO2 structural model of enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
mutant E47A, 37°C, pH 7.6 Escherichia coli
2
-
mutant E47Q, 37°C, pH 7.6 Escherichia coli
20
-
wild-type, 37°C, pH 7.6 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Escherichia coli 2-acetolactate + CO2
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0003
-
sulfometuron methyl mutant E47Q, 37°C, pH 7.6 Escherichia coli
0.0006
-
sulfometuron methyl mutant E47A, 37°C, pH 7.6 Escherichia coli
0.0008
-
sulfometuron methyl wild-type, 37°C, pH 7.6 Escherichia coli
0.0032
-
sulfometuron methyl mutant D428N, 37°C, pH 7.6 Escherichia coli
0.006
-
sulfometuron methyl mutant D428E, 37°C, pH 7.6 Escherichia coli