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Literature summary for 2.2.1.6 extracted from

  • Maier, W.; Luthra, R.; Gröger, D.
    Acetohydroxyacid synthase from Claviceps purpurea: partial purification and characterization (1989), J. Basic Microbiol., 29, 483-490.
No PubMed abstract available
Search for more literature for 2.2.1.6

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
 Claviceps purpurea
PCMB
-
 Claviceps purpurea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17
-
 pyruvate
-
 Claviceps purpurea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mn2+ or Mg2+ required Claviceps purpurea
Mn2+
-
 Claviceps purpurea

Organism

Organism UniProt Comment Textmining
Claviceps purpurea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Claviceps purpurea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.135
-
-
 Claviceps purpurea

Storage Stability

Storage Stability Organism
-25°C, no significant loss of activity after 6 months Claviceps purpurea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Claviceps purpurea 2-acetolactate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Claviceps purpurea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life: 88 seconds Claviceps purpurea
70
-
 half-life: 65 seconds Claviceps purpurea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Claviceps purpurea

Cofactor

Cofactor Comment Organism Structure
FAD no requirement Claviceps purpurea
thiamine diphosphate required as cofactor Claviceps purpurea
thiamine diphosphate Km: 0.0012 mM Claviceps purpurea