Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | stimulates | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | inhibits activity of enzyme assayed in acetate buffer | Bacillus subtilis | |
SO42- | competitive when assayed in phosphate buffer, mixed type, when assayed in acetate buffer | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
pyruvate | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Bacillus subtilis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.28 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Bacillus subtilis | 2-acetolactate + CO2 | - |
? | |
pyruvate | - |
Bacillus subtilis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
acetate buffer | Bacillus subtilis |
7 | - |
phosphate buffer | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 55% of maximal activity, pH 8.0: about 50% of maximal activity | Bacillus subtilis |