Cloned (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | Km: 0.022 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9800 | - |
2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
59000 | - |
2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
140000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
isoenzyme II, recombinantly produced in Escherichia coli HB101/pDU9 | - |
Oxidation Stability | Organism |
---|---|
oxygen-dependent inactivation of the enzyme complexed with thiamine diphosphate, FAD and magnesium acetolactate, no inactivation of the enzyme-FAD complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Comment) | Organism |
---|---|
isoenzyme II, recombinantly produced | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-80°C, concentrated enzyme solution is indefinitely stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-acetolactate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains bound FAD, 0.88 mol per mol of protomer of 69000 Da | Salmonella enterica subsp. enterica serovar Typhimurium | |
thiamine diphosphate | required as cofactor | Salmonella enterica subsp. enterica serovar Typhimurium | |
thiamine diphosphate | Km: 0.0015 mM | Salmonella enterica subsp. enterica serovar Typhimurium |