BRENDA - Enzyme Database show
show all sequences of 2.2.1.5

The synergistic decarboxylation of glyoxylate and 2-oxoglutarate by an enzyme system from pig-liver mitochondria

Steward, P.R.; Quale, J.R.; Biochem. J. 102, 885-897 (1967)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Avidin
-
Sus scrofa
mercaptoethanol
stimulates
Sus scrofa
sucrose
stimulates
Sus scrofa
General Stability
General Stability
Organism
mercaptoethanol stabilises
Sus scrofa
Mg2+ stabilises
Sus scrofa
sucrose stabilises
Sus scrofa
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 10 mM
Sus scrofa
N-ethylmaleimide
74% inhibition at 0.01 mM
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
glyoxylate
-
Sus scrofa
16
-
2-oxoglutarate
-
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Sus scrofa
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cl-
stimulates
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
pig
-
Oxidation Stability
Oxidation Stability
Organism
access of air destabilises
Sus scrofa
Purification (Commentary)
Commentary
Organism
partial
Sus scrofa
Reaction
Reaction
Commentary
Organism
2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2
various alternative mechanisms proposed
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
-
-
Sus scrofa
Storage Stability
Storage Stability
Organism
-20°C, potassium phosphate buffer, pH 7.0, 5 days
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + glyoxylate
-
395930
Sus scrofa
2-hydroxy-3-oxohexandioate + CO2
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Sus scrofa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
-
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Sus scrofa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Avidin
-
Sus scrofa
mercaptoethanol
stimulates
Sus scrofa
sucrose
stimulates
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thiamine diphosphate
-
Sus scrofa
General Stability (protein specific)
General Stability
Organism
mercaptoethanol stabilises
Sus scrofa
Mg2+ stabilises
Sus scrofa
sucrose stabilises
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
complete inhibition at 10 mM
Sus scrofa
N-ethylmaleimide
74% inhibition at 0.01 mM
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
glyoxylate
-
Sus scrofa
16
-
2-oxoglutarate
-
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Sus scrofa
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cl-
stimulates
Sus scrofa
Oxidation Stability (protein specific)
Oxidation Stability
Organism
access of air destabilises
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
85
-
-
Sus scrofa
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, potassium phosphate buffer, pH 7.0, 5 days
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + glyoxylate
-
395930
Sus scrofa
2-hydroxy-3-oxohexandioate + CO2
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Sus scrofa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
-
Sus scrofa
Other publictions for EC 2.2.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736420
Balakrishnan
Influence of allosteric regula ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
288
21688-21702
2013
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1
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2
2
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1
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2
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161
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1
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2
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2
1
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1
2
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1
1
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2
2
2
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1
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2
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1
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2
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2
1
-
-
-
-
-
-
-
2
2
736654
Patel
-
Investigation of the donor and ...
Escherichia coli
J. Mol. Catal. B
98
42-45
2013
-
1
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1
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9
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1
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9
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-
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708099
de Carvalho
Activity-based metabolomic pro ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Chem. Biol.
17
323-332
2010
-
-
-
-
-
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1
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4
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3
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8
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1
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1
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1
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4
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8
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719051
Zhao
A protecting group-free synthe ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
20
6472-6474
2010
-
-
-
-
-
-
2
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1
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2
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2
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657709
Dills
-
High pressure liquid chromatog ...
Bos taurus
Biochem. Arch.
15
279-284
1999
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
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1
1
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1
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1
1
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1
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395931
Rattan
Oxalate metabolism in magnesiu ...
Rattus norvegicus
Magnes. Res.
6
127-133
1993
-
1
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
2
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1
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1
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1
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1
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1
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1
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-
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1
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-
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-
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2
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
395936
Bats
Investigations into the effect ...
Rattus norvegicus
Nephron
57
460-469
1991
-
-
-
-
-
-
-
2
1
-
-
1
-
2
-
-
1
-
-
-
1
1
2
-
1
-
-
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1
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1
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1
-
-
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-
2
1
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1
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1
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1
1
2
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
395933
Sidhu
Oxalate metabolism in thiamine ...
Rattus norvegicus
Ann. Nutr. Metab.
31
354-361
1987
-
1
-
-
-
-
-
-
1
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-
-
-
2
-
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2
1
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1
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1
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1
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1
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1
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2
1
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1
-
-
-
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-
-
-
-
-
395924
O'Fallon
Cellular localization of alpha ...
Oryctolagus cuniculus, Rattus norvegicus
Biochim. Biophys. Acta
499
321-328
1977
-
-
-
-
-
-
-
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2
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2
-
5
-
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-
4
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4
-
2
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2
-
-
2
-
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2
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2
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2
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4
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4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
395925
Zaror-Behrens
-
Further studies on 2-oxoglutar ...
Tetrahymena pyriformis
Int. J. Biochem.
7
111-116
1976
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
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-
3
-
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-
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-
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1
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1
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1
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1
-
-
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
395926
Zaror-Behrens
Demonstration of 2-oxoglutarat ...
Tetrahymena pyriformis
Can. J. Biochem.
51
1505-1508
1973
-
-
-
-
-
-
1
-
1
2
-
-
-
2
-
-
-
-
-
-
2
-
2
-
1
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1
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1
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1
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1
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1
2
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2
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2
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1
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1
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349030
Hirabayashi
-
Inhibition of the alpha-ketogl ...
Saccharomyces cerevisiae
Agric. Biol. Chem.
36
1249-1251
1972
-
-
-
-
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1
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1
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2
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1
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1
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1
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1
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1
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2
-
1
-
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1
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395935
Prather
-
Glycine and glyoxylate decarbo ...
Nicotiana rustica
Phytochemistry
11
1637-1647
1972
-
-
-
-
-
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-
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1
2
-
1
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1
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1
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2
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1
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1
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1
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1
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1
2
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1
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1
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2
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1
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1
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-
-
-
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-
-
-
-
395927
Yamasaki
Purification, general properti ...
Mycolicibacterium phlei
Biochim. Biophys. Acta
242
637-647
1971
-
-
-
-
-
-
4
2
-
2
-
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2
-
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1
1
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1
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3
-
1
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2
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1
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1
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4
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2
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2
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1
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1
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3
-
1
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2
-
-
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395928
Schlossberg
Carboligase activity of alpha- ...
Bos taurus, Rattus norvegicus
Biochemistry
9
1148-1153
1970
-
-
-
-
-
-
-
-
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2
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1
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9
1
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4
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2
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1
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2
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2
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1
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9
1
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4
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2
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1
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-
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395932
Yamasaki
alpha-Ketoglutarate:glyoxylate ...
Escherichia coli
Biochem. Biophys. Res. Commun.
39
790-795
1970
1
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1
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1
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1
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395929
Schlossberg
Isolation and identification o ...
Bos taurus
Biochemistry
7
333-337
1968
-
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
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395930
Steward
The synergistic decarboxylatio ...
Sus scrofa
Biochem. J.
102
885-897
1967
3
-
-
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3
2
2
1
1
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1
1
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1
1
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1
1
1
1
-
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1
1
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1
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3
-
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1
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3
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2
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2
1
1
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1
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1
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1
1
1
1
-
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1
1
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