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Literature summary for 2.2.1.3 extracted from

  • Ro, Y.T.; Eom, C.Y.; Song, T.; Cho, J.W.; Kim, Y.M.
    Dihydroxyacetone synthase from a methanol-utilizing carboxydobacterium, Acinetobacter sp. strain JC1 DSM 3803 (1997), J. Bacteriol., 179, 6041-6047.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.2.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0333
-
 D-xylulose 5-phosphate
-
 Acinetobacter sp.
1.86
-
 formaldehyde
-
 Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
 alpha2, 2 * 73000, SDS-PAGE Acinetobacter sp.
140000
-
 gel filtration Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
 JC1 DSM 3803
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12
-
-
 Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + D-xylulose 5-phosphate
-
 Acinetobacter sp. glyceraldehyde 3-phosphate + 1,3-dihydroxybutane-2-one
-
 ?
D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
 Acinetobacter sp. D-fructose 6-phosphate + glyceraldehyde 3-phosphate
-
 ?
D-ribose 5-phosphate + formaldehyde
-
 Acinetobacter sp. ?
-
 ?
D-xylulose 5-phosphate + D-glyceraldehyde
-
 Acinetobacter sp. glyceraldehyde 3-phosphate + D-xylulose
-
 ?
fructose 6-phosphate + formaldehyde
-
 Acinetobacter sp. dihydroxyacetone + erythrose 4-phosphate
-
 ?
glucose 6-phosphate + formaldehyde
-
 Acinetobacter sp. ?
-
 ?
glycolaldehyde + D-xylulose 5-phosphate
-
 Acinetobacter sp. glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
-
 ?
hydroxypyruvate + formaldehyde
-
 Acinetobacter sp. CO2 + dihydroxyacetone
-
 ?
ribulose 5-phosphate + formaldehyde
-
 Acinetobacter sp. glyceraldehyde 3-phosphate + 1,3-dihydroxyacetone
-
 ?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 73000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 rapidly inactivated Acinetobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7
-
-
 Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate requirement, thiamine diphosphate protein, 0.5 mM Acinetobacter sp.