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Literature summary for 2.2.1.3 extracted from
- Dihydroxyacetone synthase: a special transketolase for formaldehyde fixation from the methylotrophic yeast Candida boidinii CBS 5777 (1983), J. Gen. Microbiol., 129, 935-944.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
[Candida] boidinii |  |
| GSH | - |
[Candida] boidinii | |
| Ni2+ | - |
[Candida] boidinii | |
| ribose | - |
[Candida] boidinii | |
| sulfate | - |
[Candida] boidinii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | [Candida] boidinii | |
| Mn2+ | activation, replaces Mg2+ to some extent | [Candida] boidinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62500 | - |
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE | [Candida] boidinii |
| 65500 | - |
alpha2, 2 * 65500, SDS-PAGE | [Candida] boidinii |
| 105000 | 110000 | gel filtration | [Candida] boidinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + formaldehyde | [Candida] boidinii | - |
glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] boidinii | - |
methanol grown | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.766 | - |
- |
[Candida] boidinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose 5-phosphate + butyraldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + 1,3-dihydroxyhexane-2-one | - |
? | |
| D-xylulose 5-phosphate + D-erythrose | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + D-fructose | - |
? | |
| D-xylulose 5-phosphate + D-glucose | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + D-glycero-D-ido-oct-2-ulose | - |
? | |
| D-xylulose 5-phosphate + D-ribose | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + sedoheptulose | - |
? | |
| D-xylulose 5-phosphate + formaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | best substrate | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | D-xylulose 5-phosphate cannot be replaced by D-ribulose 5-phosphate, D-glucose 6-phosphate, or D-ribose 5-phosphate | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + formaldehyde | no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone | [Candida] boidinii | glyceraldehyde 3-phosphate + dihydroxyacetone | i.e. 1,3-dihydroxy-2-propanone | ? | |
| D-xylulose 5-phosphate + glycolaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one | - |
? | |
| D-xylulose 5-phosphate + heptaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + 1,3-dihydroxynonane-2-one | - |
? | |
| D-xylulose 5-phosphate + propionaldehyde | - |
[Candida] boidinii | glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one | - |
? | |
| fructose 6-phosphate + formaldehyde | fructose 6-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor | [Candida] boidinii | dihydroxyacetone + erythrose 4-phosphate | - |
? | |
| hydroxypyruvate + formaldehyde | hydroxypyruvate can replace D-xylulose 5-phosphate with 41.4%, hydroxypyruvate can replace D-xylulose 5-phosphate with 56% | [Candida] boidinii | CO2 + dihydroxyacetone | - |
? | |
| sedoheptulose 7-phosphate + formaldehyde | sedoheptulose 7-phosphate can replace D-xylulose 5-phosphate with 10% efficiency as glycolaldehyde donor | [Candida] boidinii | dihydroxyacetone + ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE | [Candida] boidinii |
| dimer | alpha2, 2 * 65500, SDS-PAGE | [Candida] boidinii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
15 min in stabilizing buffer | [Candida] boidinii |
| 35 | - |
5 min without stabilizing agents | [Candida] boidinii |
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