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Literature summary for 2.2.1.3 extracted from

  • Bystrykh, L.V.; Sokolov, A.P.; Trotsenko, Y.A.
    Purification and properties of dihydroxyacetone synthase from methylotrophic yeast Candida boidinii (1981), FEBS Lett., 132, 324-328.
No PubMed abstract available

General Stability

General Stability Organism
Ca2+ does not stabilize [Candida] boidinii
Mg2+ or NH4+ stabilizes for at least 80 h [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
[Candida] boidinii
formaldehyde above 1 mM, xylulose 5-phosphate protects [Candida] boidinii
GSH
-
[Candida] boidinii
NEM irreversible, GSH partially protects [Candida] boidinii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule [Candida] boidinii
additional information no activation by Ca2+ [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
alpha2, 2 * 76000, SDS-PAGE [Candida] boidinii
145000
-
gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylulose 5-phosphate + formaldehyde [Candida] boidinii
-
glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde [Candida] boidinii KD1
-
glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-
[Candida] boidinii
-
methanol grown
-
[Candida] boidinii KD1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4
-
-
[Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + formaldehyde
-
[Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde formaldehyde is the only acceptor [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone [Candida] boidinii glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde
-
[Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde formaldehyde is the only acceptor [Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
D-xylulose 5-phosphate + formaldehyde no acceptors are glyceraldehyde, glyceraldehyde 3-phosphate, erythrose 4-phosphate, ribose 5-phosphate, glucose 6-phosphate, D-ribose, glutaraldehyde, dihydroxyacetone [Candida] boidinii KD1 glyceraldehyde 3-phosphate + dihydroxyacetone i.e. 1,3-dihydroxy-2-propanone ir
fructose 6-phosphate + formaldehyde
-
[Candida] boidinii dihydroxyacetone + erythrose 4-phosphate
-
?
fructose 6-phosphate + formaldehyde
-
[Candida] boidinii KD1 dihydroxyacetone + erythrose 4-phosphate
-
?
sedoheptulose 7-phosphate + formaldehyde
-
[Candida] boidinii dihydroxyacetone + ribose
-
?
sedoheptulose 7-phosphate + formaldehyde
-
[Candida] boidinii KD1 dihydroxyacetone + ribose
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 76000, SDS-PAGE [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
[Candida] boidinii
6.8 7.1
-
[Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate tightly bound [Candida] boidinii
thiamine diphosphate requirement, thiamine diphosphate protein, 0.5 mM [Candida] boidinii