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Literature summary for 2.2.1.2 extracted from
- Purification, characterization and mass spectrometric sequencing of transaldolase from Fusarium oxysporum (2008), Process Biochem., 43, 1094-1101.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
D-erythrose-4-phosphate | - |
Fusarium oxysporum |  |
| 6.66 | - |
D-fructose-6-phosphate | - |
Fusarium oxysporum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
SDS-PAGE, denatured | Fusarium oxysporum |
| 38000 | - |
SDS-PAGE, purified | Fusarium oxysporum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium oxysporum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange and gel filtration chromatographies, after last step 50fold purification | Fusarium oxysporum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
crude enzyme | Fusarium oxysporum |
| 15 | - |
enzyme after purification with anion exchange and gel filtration chromatography | Fusarium oxysporum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 30°C, 1 h, 20% activity lost | Fusarium oxysporum |
| 40°C, 1 h, 40% activity lost | Fusarium oxysporum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate + D-erythrose 4-phosphate | assay at 25°C, pH 7.5 | Fusarium oxysporum | glyceraldehyde 3-phosphate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Fusarium oxysporum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FoTal | - |
Fusarium oxysporum |
| transaldolase | - |
Fusarium oxysporum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Fusarium oxysporum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | at 30°C for 1 h only 80% residual activity, at 40°C for 1 h only 60% residual activity, at 50°C enzyme rapidly inactive, at 55°C about 30% activity lost | Fusarium oxysporum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
imidazole buffer | Fusarium oxysporum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 80% loss of activity with Tris/HCl buffer at pH 7.5, 95% loss of activity with Tris/HCl buffer at pH 9.0, 62% loss of activity with glycine/NaOH buffer at pH 9.0, about half of enzyme activity lost with phosphate buffer at pH 6.0 | Fusarium oxysporum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusarium oxysporum | isoelectric focusing-PAGE | - |
4.5 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.38 | - |
D-fructose-6-phosphate | - |
Fusarium oxysporum | |
| 139.9 | - |
D-erythrose-4-phosphate | - |
Fusarium oxysporum | |
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