Literature summary for 2.2.1.2 extracted from

Fiki, A.E.; Metabteb, G.E.; Bellebna, C.; Wartmann, T.; Bode, R.; Gellissen, G.; Kunze, G.
The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation (2007), Appl. Microbiol. Biotechnol., 74, 1292-1299.

Search for more literature for 2.2.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
NaCl	increased expression in media containing 5% NaCl	Blastobotrys adeninivorans

Cloned(Commentary)

Cloned (Comment)	Organism
-	Blastobotrys adeninivorans
expressed in Escherichia coli	Blastobotrys adeninivorans
gene ATAL, DNA and amino acid sequence determination and analysis, including promoter and terminator sequences, single copy gene, genetic organization, expression in Escherichia coli	Blastobotrys adeninivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.013	-	D-erythrose-4-phosphate	-	Blastobotrys adeninivorans
0.013	-	D-erythrose 4-phosphate	pH 6.0, 20°C	Blastobotrys adeninivorans
0.056	-	D-fructose 6-phosphate	-	Blastobotrys adeninivorans
0.056	-	D-fructose 6-phosphate	pH 6.0, 20°C	Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	denatured enzyme, SDS-PAGE	Blastobotrys adeninivorans
35000	-	4 * 35000, SDS-PAGE	Blastobotrys adeninivorans
35095	-	4 * 35000, SDS-PAGE, 4 * 35095, calculated	Blastobotrys adeninivorans
140000	-	gel filtration	Blastobotrys adeninivorans
140000	-	native enzyme (tetramer), gel filtration	Blastobotrys adeninivorans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-erythrose 4-phosphate + D-fructose 6-phosphate	Blastobotrys adeninivorans	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-erythrose 4-phosphate + D-fructose 6-phosphate	Blastobotrys adeninivorans LS3	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Blastobotrys adeninivorans	-	-	-
Blastobotrys adeninivorans	-	increased expression in media containing 5% NaCl	-
Blastobotrys adeninivorans	Q0E4W0	gene ATAL	-
Blastobotrys adeninivorans LS3	Q0E4W0	gene ATAL	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-erythrose 4-phosphate + D-fructose 6-phosphate	-	Blastobotrys adeninivorans	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-erythrose 4-phosphate + D-fructose 6-phosphate	the preferred substrates for the enzyme include D-erythrose-4-phosphate and D-fructose-6-phosphate, whereas D-glyceraldehyde is not converted	Blastobotrys adeninivorans	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-erythrose 4-phosphate + D-fructose 6-phosphate	-	Blastobotrys adeninivorans LS3	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-erythrose 4-phosphate + D-fructose 6-phosphate	the preferred substrates for the enzyme include D-erythrose-4-phosphate and D-fructose-6-phosphate, whereas D-glyceraldehyde is not converted	Blastobotrys adeninivorans LS3	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 6-phosphate + D-erythrose 4-phosphate	preferred substrates	Blastobotrys adeninivorans	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
additional information	no substrate: D-glyceraldehyde	Blastobotrys adeninivorans	?	-	?
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Blastobotrys adeninivorans	D-erythrose 4-phosphate + D-fructose 6-phosphate	-	r

Subunits

Subunits	Comment	Organism
tetramer	-	Blastobotrys adeninivorans
tetramer	4 * 35000, SDS-PAGE	Blastobotrys adeninivorans
tetramer	4 * 35000, SDS-PAGE, 4 * 35095, calculated	Blastobotrys adeninivorans

Synonyms

Synonyms	Comment	Organism
ATAL	-	Blastobotrys adeninivorans
Atalp	-	Blastobotrys adeninivorans
transaldolase	-	Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	-	Blastobotrys adeninivorans
20	-	native and recombinant enzyme	Blastobotrys adeninivorans

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
18	25	-	Blastobotrys adeninivorans
18	25	native and recombinant enzyme, 80% of maximal activity within this range	Blastobotrys adeninivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	D-erythrose 4-phosphate	pH 6.0, 20°C	Blastobotrys adeninivorans
4.9	-	D-fructose 6-phosphate	-	Blastobotrys adeninivorans
4.9	-	D-fructose 6-phosphate	pH 6.0, 20°C	Blastobotrys adeninivorans
6.44	-	D-erythrose-4-phosphate	-	Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	native enzyme	Blastobotrys adeninivorans
5.5	-	-	Blastobotrys adeninivorans
5.5	-	native enzyme	Blastobotrys adeninivorans
6	-	recombinant enzyme	Blastobotrys adeninivorans

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	6	-	Blastobotrys adeninivorans
5	6	native enzyme, 80% of maximal activity within this range	Blastobotrys adeninivorans
5	6	native enzyme	Blastobotrys adeninivorans
5.4	6.3	recombinant enzyme	Blastobotrys adeninivorans
5.4	6.3	recombinant enzyme, 80% of maximal activity within this range	Blastobotrys adeninivorans

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Release 2023.1 (January 2023)