Cloned (Comment) | Organism |
---|---|
gene Mj0400, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | Methanocaldococcus jannaschii | 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57843 | gene mj0400 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate and L-aspartate semialdehyde are precursors to 3-dehydroquinate, DHQ | Methanocaldococcus jannaschii | ? | - |
? | |
L-aspartate semialdehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate | the MJ0400 gene product catalyzes the transaldolase reaction | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | GC-MS analysis of reaction intermediates, overview. The enzyme is active as aldose with erythrose 4-phosphate and pyruvate producing a different stereoisomer from 3-deoxy-D-arabino-2-heptulosonate-7-phosphate | Methanocaldococcus jannaschii | ? | - |
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Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
metabolism | Mj0400 is involved in the biosynthesis of aromatic compounds, including 3-dehydroquinate via an undetermined intermediate and 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid, which is formed by NAD+-dependent Mj1249 from the intermediate compound. Also 4,5-dihydroxy-6-methylpipecolinic acid is formed from the intermediate. Pathway analysis, detailed overview | Methanocaldococcus jannaschii |