Literature summary for 2.2.1.1 extracted from

Markert, B.; Stolzenberger, J.; Brautaset, T.; Wendisch, V.F.
Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus (2014), BMC Microbiol., 14, 7.

Search for more literature for 2.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus methanolicus

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Bacillus methanolicus
ATP	-	Bacillus methanolicus
EDTA	-	Bacillus methanolicus
Ni2+	-	Bacillus methanolicus
phosphate buffer	20 mM phosphate buffer shows an inhibitory effect of the enzyme activity of approximately 40%	Bacillus methanolicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	D-ribose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
0.15	-	D-xylulose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
0.23	-	D-xylulose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
0.25	-	D-fructose 6-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
0.25	-	D-ribose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
0.67	-	D-glyceraldehyde 3-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
0.72	-	D-fructose 6-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
0.92	-	D-glyceraldehyde 3-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Bacillus methanolicus
Co2+	activates	Bacillus methanolicus
Cu2+	activates	Bacillus methanolicus
Mg2+	activates	Bacillus methanolicus
Mn2+	activates	Bacillus methanolicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73000	-	4 * 73000, SDS-PAGE	Bacillus methanolicus
280000	-	gel filtration	Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate	Bacillus methanolicus	-	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?
D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate	Bacillus methanolicus MGA3	-	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?
D-ribose 5-phosphate + D-xylulose 5-phosphate	Bacillus methanolicus	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-ribose 5-phosphate + D-xylulose 5-phosphate	Bacillus methanolicus MGA3	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus methanolicus	-	-	-
Bacillus methanolicus MGA3	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Bacillus methanolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate	-	Bacillus methanolicus	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?
D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate	-	Bacillus methanolicus MGA3	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?
D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Bacillus methanolicus	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?
D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Bacillus methanolicus MGA3	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 73000, SDS-PAGE	Bacillus methanolicus

Synonyms

Synonyms	Comment	Organism
TKT	-	Bacillus methanolicus
TKTc	isoform	Bacillus methanolicus
TKTp	isoform	Bacillus methanolicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
62	-	-	Bacillus methanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	the enzyme isoforms TKTc and TKTp remain stable up to 50°C for at least 2 h. Upon pre-incubation at 60°C the catalytic activity is reduced for both enzymes to approximately 60% within 10 min and then remains stable at this level. Incubation at 70°C leads to a complete loss of activity for TKTc after 4 min, for TKTp after 30 min of incubation	Bacillus methanolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
13	-	D-ribose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
21	-	D-ribose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
40	-	D-xylulose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
48	-	D-glyceraldehyde 3-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
54	-	D-xylulose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
82	-	D-fructose 6-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
99	-	D-glyceraldehyde 3-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
112	-	D-fructose 6-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	7.4	-	Bacillus methanolicus

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Bacillus methanolicus

General Information

General Information	Comment	Organism
metabolism	transketolase is a key enzyme of the pentose phosphate pathway, the Calvin cycle and the ribulose monophosphate cycle	Bacillus methanolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
71	-	D-glyceraldehyde 3-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
84	-	D-ribose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
108	-	D-glyceraldehyde 3-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
109	-	D-ribose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
115	-	D-fructose 6-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
231	-	D-xylulose 5-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus
264	-	D-xylulose 5-phosphate	isoform TKTc, at pH 7.5 and 55°C	Bacillus methanolicus
448	-	D-fructose 6-phosphate	isoform TKTp, at pH 7.7 and 55°C	Bacillus methanolicus

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Release 2023.1 (January 2023)