Literature summary for 2.2.1.1 extracted from

Solovjeva, O.N.; Sevostyanova, I.A.; Yurshev, V.A.; Selivanov, V.A.; Kochetov, G.A.
Effects of free Ca2+ on kinetic characteristics of holotransketolase (2012), Protein J., 31, 137-140.

Search for more literature for 2.2.1.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.007	-	D-ribose 5-phosphate	and second value 0.698 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication	Saccharomyces cerevisiae
0.025	-	D-xylulose 5-phosphate	and second value 0.773 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication	Saccharomyces cerevisiae
0.075	-	D-xylulose 5-phosphate	pH 7.6, temperature not specified in the publication	Saccharomyces cerevisiae
0.159	-	D-ribose 5-phosphate	pH 7.6, temperature not specified in the publication	Saccharomyces cerevisiae
0.698	-	D-ribose 5-phosphate	and first value 0.007 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication	Saccharomyces cerevisiae
0.773	-	D-xylulose 5-phosphate	and first value 0.025 mM, pH 7.6, presence of 0.1 mM Ca2+, temperature not specified in the publication	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	in the presence of free Ca2+, the equivalence of the two active centers of the enzyme is lost. Negative cooperativity is induced on binding of either xylulose 5-phosphate or ribose 5-phosphate, whereupon the catalytic conversion of the bound substrates causes the interaction between the centers to become positively cooperative. Enzyme total activity increases in presence of Ca2+	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate	-	Saccharomyces cerevisiae	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)