Literature summary for 2.2.1.1 extracted from

Naula, C.; Alibu, V.P.; Brock, J.M.; Veitch, N.J.; Burchmore, R.J.; Barrett, M.P.
A new erythrose 4-phosphate dehydrogenase coupled assay for transketolase (2008), J. Biochem. Biophys. Methods, 70, 1185-1187.

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Application

Application	Comment	Organism
analysis	coupling of a transketolase reaction that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using erythrose-4-phosphate dehydrogenase, a reaction that reduces NAD+ to NADH and can be easily followed spectrophotometrically	Leishmania mexicana

Cloned(Commentary)

Cloned (Comment)	Organism
-	Leishmania mexicana

Inhibitors

Inhibitors	Comment	Organism	Structure
fructose 6-phosphate	at concentrations higher than 10 mM fructose 6-phosphate causes an inhibition of the enzyme	Leishmania mexicana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.61	-	fructose 6-phosphate	-	Leishmania mexicana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Leishmania mexicana

Organism

Organism	UniProt	Comment	Textmining
Leishmania mexicana	Q8MPM3	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.8	-	using 10 mM D-glyceraldehyde 3-phosphate	Leishmania mexicana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate	-	Leishmania mexicana	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	?
D-xylulose 5-phosphate + D-ribose 5-phosphate	-	Leishmania mexicana	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TKT	-	Leishmania mexicana

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Leishmania mexicana

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Release 2023.1 (January 2023)