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Literature summary for 2.2.1.1 extracted from
- Asp477 is a determinant of the enantioselectivity in yeast transketolase (1998), FEBS Lett., 424, 49-52.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D477A | lower activity than wild-type enzyme | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-erythrose 4-phosphate + ? | - |
Saccharomyces cerevisiae | ? | - |
? |  |
| 2-deoxy-D-ribose 5-phosphate + ? | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-arabinose 5-phosphate + ? | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-erythrose 4-phosphate + ? | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-threose 4-phosphate + ? | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
D-arabinose 5-phosphate | D477A | Saccharomyces cerevisiae | |
| 0.07 | - |
D-threose 4-phosphate | D477A | Saccharomyces cerevisiae | |
| 0.3 | - |
2-deoxy-D-ribose 5-phosphate | wild-type | Saccharomyces cerevisiae | |
| 0.3 | - |
D-threose 4-phosphate | wild-type | Saccharomyces cerevisiae | |
| 0.4 | - |
D-arabinose 5-phosphate | wild-type | Saccharomyces cerevisiae | |
| 0.4 | - |
2-deoxy-D-ribose 5-phosphate | D477A | Saccharomyces cerevisiae | |
| 0.8 | - |
D-ribose 5-phosphate | D477A | Saccharomyces cerevisiae | |
| 1.3 | - |
2-deoxy-D-erythrose 4-phosphate | D477A | Saccharomyces cerevisiae | |
| 1.4 | - |
D-erythrose 4-phosphate | D477A | Saccharomyces cerevisiae | |
| 4.9 | - |
2-deoxy-D-erythrose 4-phosphate | wild-type | Saccharomyces cerevisiae | |
| 46 | - |
D-ribose 5-phosphate | wild-type | Saccharomyces cerevisiae | |
| 69 | - |
D-erythrose 4-phosphate | wild-type | Saccharomyces cerevisiae | |
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