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Literature summary for 2.2.1.1 extracted from

  • Soh, Y.; Song, B.J.; Jeng, J.; Kallarakal, A.T.
    Critical role of Arg433 in rat transketolase activity as probed by site-directed mutagenesis (1998), Biochem. J., 333, 367-372.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R102A similar catalytyc activity like wild-type enzyme Rattus norvegicus
R350A lower activity than wild-type enzyme Rattus norvegicus
R433A lower activity than wild-type enzyme, less stable at 55°C than wild-type Rattus norvegicus
R506A lower activity than wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0362
-
D-ribose 5-phosphate wild-type Rattus norvegicus
0.0399
-
D-xylulose 5-phosphate wild-type Rattus norvegicus
0.4579
-
D-xylulose 5-phosphate R433A Rattus norvegicus
0.879
-
D-ribose 5-phosphate R433A Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
alpha2 2 * 74000, SDS-PAGE Rattus norvegicus
148000
-
PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Subunits

Subunits Comment Organism
dimer alpha2 2 * 74000, SDS-PAGE Rattus norvegicus