Literature summary for 2.2.1.1 extracted from

Nilsson, U.; Meshalkina, L.; Lindqvist, Y.; Schneider, G.
Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis (1997), J. Biol. Chem., 272, 1864-1869.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
cocrystallization of apotransketolase with 5 mM thiamine diphosphate, 5 mM CaCl2, 50 mM fructose-6-phosphate, 13-16% (w/W) polyethylenglycol 6000 in 50 mM glycyl-glycine buffer, pH 7.6, 0.0075 ml of a 20 mg/ml solution mixed with the same amount of mother liquid, space group: P212121	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
D477A	lower activity than wild-type enzyme	Saccharomyces cerevisiae
H469A	lower activity than wild-type enzyme	Saccharomyces cerevisiae
R359A	lower activity than wild-type enzyme	Saccharomyces cerevisiae
R528A	lower activity than wild-type enzyme	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0005	-	thiamine diphosphate	H469A	Saccharomyces cerevisiae
0.0006	-	thiamine diphosphate	wild-type	Saccharomyces cerevisiae
0.0012	-	thiamine diphosphate	D477A	Saccharomyces cerevisiae
0.0019	-	thiamine diphosphate	R359A	Saccharomyces cerevisiae
0.0024	-	thiamine diphosphate	R528A	Saccharomyces cerevisiae
0.073	-	D-xylulose 5-phosphate	wild-type	Saccharomyces cerevisiae
0.146	-	D-ribose 5-phosphate	wild-type	Saccharomyces cerevisiae
0.163	-	D-xylulose 5-phosphate	R359A	Saccharomyces cerevisiae
0.318	-	D-xylulose 5-phosphate	R528A	Saccharomyces cerevisiae
0.595	-	D-xylulose 5-phosphate	D477A	Saccharomyces cerevisiae
0.829	-	D-xylulose 5-phosphate	H469A	Saccharomyces cerevisiae
1.75	-	D-ribose 5-phosphate	D477A	Saccharomyces cerevisiae
5.65	-	D-ribose 5-phosphate	R359A	Saccharomyces cerevisiae
5.97	-	D-ribose 5-phosphate	H469A	Saccharomyces cerevisiae
7	-	D-ribose 5-phosphate	R528A	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74200	-	alpha2, 2 * 74200, SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P23254	baker's yeast	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate	-	Saccharomyces cerevisiae	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	alpha2, 2 * 74200, SDS-PAGE	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.2	7.8	R359A, R528A	Saccharomyces cerevisiae
6.6	7.8	H469A	Saccharomyces cerevisiae
6.8	7.8	D477A	Saccharomyces cerevisiae
7.6	8.2	wild-type	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)