General Stability | Organism |
---|---|
glycerol, 10% v/v, stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Rabbit antibodies | not | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
D-xylulose 5-phosphate | (+ D-ribose 5-phosphate) | Rattus norvegicus | |
0.72 | - |
D-ribose 5-phosphate | cosubstrate D-xylulose 5-phosphate | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
139000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | Rattus norvegicus | - |
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immunoaffinity chromatography | Rattus norvegicus |
partial, 5 multiple forms by isoelectric focusing | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, more than a week | Rattus norvegicus |
-20°C, up to 2 months with 10% v/v glycerol | Rattus norvegicus |
0-4°C, several h | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Rattus norvegicus | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 69000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
45 | - |
assay at, low enzyme concentration | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.2 | about half-maximal activity at pH 6.5 and 10.2 | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
below, unstable | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Rattus norvegicus |