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Literature summary for 2.2.1.1 extracted from
- Characterization and properties of pig liver transketolase (1980), Biochim. Biophys. Acta, 613, 359-369.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Sus scrofa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
| 54000 | - |
alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
| 138000 | - |
meniscus depletion method | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.88 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several days | Sus scrofa |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2beta2, 2 * 30000 + 2 * 54000, pretreated with 1% SDS, gel filtration in the presence of 0.1% SDS | Sus scrofa |
| tetramer | alpha2beta2, 2 * 27000-29000 + 2 * 52000-56000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Sus scrofa |
| 30 | - |
assay at | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
at pH 6.5-8.7, 20 min stable | Sus scrofa |
| 40 | - |
pH 8.0, activity hardly changes for 60 min | Sus scrofa |
| 50 | - |
t1/2: 10 min | Sus scrofa |
| 50 | - |
1 h stable in the presence of 4 mM thiamine diphosphate | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 7.6-7.8 | Sus scrofa |
| 7.8 | 8.2 | - |
Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
thiamine diphosphate dissociates from holoenzyme | Sus scrofa |
| 6.5 | 8.7 | 20°C, 20 min stable | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | 2 mol thiamine diphosphate per mol enzyme | Sus scrofa |  |
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