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Literature summary for 2.2.1.1 extracted from

  • Demuynck, C.; Fisson, F.; Bennani-Baiti, I.; Samaki, H.; Mani, J.C.
    Immunoaffinity purification of transketolases from yeast and spnich leaves (1990), Agric. Biol. Chem., 54, 3073-3078.
No PubMed abstract available
Search for more literature for 2.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Rabbit antibodies polyclonal Saccharomyces cerevisiae
Rabbit antibodies not Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Saccharomyces cerevisiae
Mg2+ requirement Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74200
-
 alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae
100000
-
 1 * 100000, SDS-PAGE Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate Saccharomyces cerevisiae pathway in non-oxidative sequence of pentose cycle sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + D-ribose 5-phosphate Spinacia oleracea pathway in non-oxidative sequence of pentose cycle sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 baker's yeast
-
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
immunoaffinity chromatography Saccharomyces cerevisiae
immunoaffinity chromatography Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
-
 Saccharomyces cerevisiae
40
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + D-ribose 5-phosphate and acceptor substrates may be: D-glyceraldehyde 3-phosphate Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + D-ribose 5-phosphate pathway in non-oxidative sequence of pentose cycle Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + D-ribose 5-phosphate pathway in non-oxidative sequence of pentose cycle Spinacia oleracea sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
hydroxypyruvate + D-glyceraldehyde 3-phosphate enantioselective Saccharomyces cerevisiae CO2 + ribulose 5-phosphate
-
 ?
hydroxypyruvate + D-glyceraldehyde 3-phosphate enantioselective Spinacia oleracea CO2 + ribulose 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Spinacia oleracea
dimer alpha2, 2 * 74200, SDS-PAGE Saccharomyces cerevisiae
monomer 1 * 100000, SDS-PAGE Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces cerevisiae
8
-
-
 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate i.e. functional form of vitamin B1 Saccharomyces cerevisiae
thiamine diphosphate i.e. functional form of vitamin B1 Spinacia oleracea
thiamine diphosphate 2 catalytic sites per enzyme molecule Saccharomyces cerevisiae
thiamine diphosphate 1 catalytic site per enzyme molecule Spinacia oleracea