Cloned (Comment) | Organism |
---|---|
PTC protomer structure, overview | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
purified full-length PTC or in complex with inhibitors N-(phosphonoacetyl)-putrescine or N-(phosphonoacetyl)-L-ornithine, and trunacted PTC in complex with N-(phosphonoacetyl)-L-ornithine, hanging drop vapor diffusion technique, mixing of 0.001 ml of 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5 containing 0.43 mM ligand with 0.001 ml of crystallization solution composed of 125 mM (NH4)2SO4, 17% PEG 3350, 0.1 M Bis-Tris, pH 5.5, 21°C, X-ray diffraction structure determination and analysis at 2.5 A, 2.0 A, and 1.59 A resolution, respectively, modeling | Enterococcus faecalis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | confirmation of decreased stability of the trimer of PTC lacking the C-terminal helix by deleting this helix | Enterococcus faecalis |
R54G | site-directed mutagenesis, inactive mutant, not exhibiting any PTC or OTC activity | Enterococcus faecalis |
Y230V/G231S/L232M/Y233G | engineering of the 230-loop of PTC, by replacing the sequence 230YGLY233 of the putrescine signature by its OTC counterpart VSMG, favors the use of ornithine and impairs that of putrescine | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(phosphonoacetyl)-putrescine | bisubstrate analogue inhibitor | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
putrescine | recombinant wild-type PTC, pH 8.5, 37°C | Enterococcus faecalis | |
32.9 | - |
putrescine | recombinant PTC mutant Y230V/G231S/L232M/Y233G, pH 8.5, 37°C | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120300 | - |
trimeric PTC, gel filtration | Enterococcus faecalis |
230000 | - |
hexameric PTC, gel filtration | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + putrescine | Enterococcus faecalis | - |
phosphate + N-carbamoylputrescine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q837U7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.13 | - |
recombinant PTC mutant Y230V/G231S/L232M/Y233G, substrate putrescine, pH 8.5, 37°C | Enterococcus faecalis |
813 | - |
recombinant wild-type PTC, substrate putrescine, pH 8.5, 37°C | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis | phosphate + N-carbamoylputrescine | - |
r | |
additional information | in addition to using putrescine, the enzyme can utilize L-ornithine as a poor substrate, see EC 2.1.3.3. Differences between the respective 230 and SMG loops of PTC and OTC appear to account for the differential preference of these enzymes for putrescine and ornithine, active center and the discrimination mechanism between putrescine and ornithine, overview | Enterococcus faecalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | two trimer structure | Enterococcus faecalis |
More | presence or absence of supratrimeric oligomerization, structure comparison and analysis, overview | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
putrescine transcarbamylase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Enterococcus faecalis |
General Information | Comment | Organism |
---|---|---|
additional information | sequence 230YGLY233 is the putrescine signature sequence | Enterococcus faecalis |
physiological function | the enzyme generates carbamoyl phosphate for ATP production in the fermentative catabolism of agmatine | Enterococcus faecalis |