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Literature summary for 2.1.3.6 extracted from

  • Chen, J.; Cheng, C.; Xia, Y.; Zhao, H.; Fang, C.; Shan, Y.; Wu, B.; Fang, W.
    Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance (2011), Microbiology, 157, 3150-3161.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview Listeria monocytogenes

Protein Variants

Protein Variants Comment Organism
additional information construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036 Listeria monocytogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + putrescine Listeria monocytogenes
-
phosphate + N-carbamoylputrescine
-
r
carbamoyl phosphate + putrescine Listeria monocytogenes 10403S
-
phosphate + N-carbamoylputrescine
-
r
additional information Listeria monocytogenes Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?
additional information Listeria monocytogenes 10403S Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions ?
-
?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
gene lmo0036
-
Listeria monocytogenes 10403S
-
gene lmo0036
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
anabolic reaction, pH 8.0, 37°C Listeria monocytogenes
35.6
-
catabolic reaction, pH 5.0, 37°C Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + putrescine
-
Listeria monocytogenes phosphate + N-carbamoylputrescine
-
r
carbamoyl phosphate + putrescine
-
Listeria monocytogenes 10403S phosphate + N-carbamoylputrescine
-
r
additional information Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes ?
-
?
additional information Lmo0036 is an ornithine carbamoyltransferase, EC 2.1.3.3, and also a putrescine carbamoyltransferase, catalysing reversible ornithine and putrescine carbamoyltransfer reactions Listeria monocytogenes 10403S ?
-
?

Synonyms

Synonyms Comment Organism
anabolic putrescine carbamoyltransferase
-
Listeria monocytogenes
aPTC
-
Listeria monocytogenes
catabolic putrescine carbamoyltransferase
-
Listeria monocytogenes
cPTC
-
Listeria monocytogenes
Lmo0036
-
Listeria monocytogenes
putrescine carbamoyltransferase
-
Listeria monocytogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Listeria monocytogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
catabolic reaction Listeria monocytogenes
8
-
anabolic reaction Listeria monocytogenes

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 activity range, catabolic reaction Listeria monocytogenes
4 11 activity range, anabolic reaction Listeria monocytogenes

General Information

General Information Comment Organism
malfunction absence of this enzyme impairs the growth of Listeria under mild acidic conditions at pH 4.8 and reduced its survival in synthetic human gastric fluid at pH 2.5, corresponding to a loss in ammonia production Listeria monocytogenes
metabolism catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of arginine deiminase and agmatine deiminase pathways. However, the equilibrium of in vitro carbamoyltransfer reactions is overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase is probably the limiting step of the pathways. lmo0036 is induced at the transcriptional level when Listeria monocytogenes is subjected to low-pH stress. Its expression product in Escherichia coli exhibits higher catabolic carbamoyltransfer activities under acidic conditions Listeria monocytogenes
physiological function Lmo0036 is responsible for acid tolerance at both sublethal and lethal pH levels and plays a possible role in Listeria virulence Listeria monocytogenes