Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.3.3 extracted from

  • Chen, L.; Li, P.; Deng, Z.; Zhao, C.
    Ornithine transcarbamylase ArgK plays a dual role for the self-defense of phaseolotoxin producing pseudomonas syringae pv. Phaseolicola (2015), Sci. Rep., 5, 12892-12901.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.3

Cloned(Commentary)

Cloned (Comment) Organism
gene argK, overexpression of His-tagged enzyme Pseudomonas savastanoi pv. phaseolicola

Protein Variants

Protein Variants Comment Organism
additional information generation of in-frame deletion mutants of ArgK Pseudomonas savastanoi pv. phaseolicola

Inhibitors

Inhibitors Comment Organism Structure
additional information Ndelta(N'-sulfo-diaminophosphinyl)-ornithyl-alanyl-homoarginine and Ndelta (N'-sulfo-diaminophosphinyl)-ornithine are irreversible inhibitor of ornithine transcarbamylase attributed to the chemical structure similarity with tetrahedral intermediates in OTCase mechanism. But the enzyme from Pseudomona syringae pv. phaseolicola shows resistance against the phytotoxic compounds.Inhibition of OTCase blocks the biosynthesis of arginine in vivo and leads to a reduction of protein synthesis Pseudomonas savastanoi pv. phaseolicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoyl phosphate + L-Orn-Ala-hArg Pseudomonas savastanoi pv. phaseolicola the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-Orn-Ala-hArg Pseudomonas savastanoi pv. phaseolicola 1448A the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-ornithine Pseudomonas savastanoi pv. phaseolicola
-
 phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine Pseudomonas savastanoi pv. phaseolicola 1448A
-
 phosphate + L-citrulline
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas savastanoi pv. phaseolicola P68747 gene argK
-
Pseudomonas savastanoi pv. phaseolicola 1448A P68747 gene argK
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Pseudomonas savastanoi pv. phaseolicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-Orn-Ala-hArg
-
 Pseudomonas savastanoi pv. phaseolicola phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-Orn-Ala-hArg the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates Pseudomonas savastanoi pv. phaseolicola phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-Orn-Ala-hArg
-
 Pseudomonas savastanoi pv. phaseolicola 1448A phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-Orn-Ala-hArg the enzyme produces Cit-Ala-hArg from tripeptide Orn-Ala-hArg and carbamyl phosphate as substrates Pseudomonas savastanoi pv. phaseolicola 1448A phosphate + L-Cit-Ala-hArg
-
 ?
carbamoyl phosphate + L-ornithine
-
 Pseudomonas savastanoi pv. phaseolicola phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Pseudomonas savastanoi pv. phaseolicola 1448A phosphate + L-citrulline
-
 ?

Synonyms

Synonyms Comment Organism
ArgK
-
 Pseudomonas savastanoi pv. phaseolicola
ornithine transcarbamylase
-
 Pseudomonas savastanoi pv. phaseolicola
otcase
-
 Pseudomonas savastanoi pv. phaseolicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Pseudomonas savastanoi pv. phaseolicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas savastanoi pv. phaseolicola

General Information

General Information Comment Organism
physiological function the enzyme ArgK in Pseudomona syringae pv. phaseolicola is resonsible for self-defense in the antimetabolic phytotoxin phaseolotoxin, a sulfodiaminophosphinyl tripeptide produced to inhibit plant host cell's ornithine transcarbamylase activity and induce arginine auxotrophic phenotype. Phaseolotoxin-resistant OTCase ArgK acts as a functional replacement of housekeeping OTCase ArgF, which is the acting target of phaseolotoxin, to confer phaseolotoxin producers with self-resistance. But neither transcriptional regulator nor thermal regulation related protein encoding gene is detected from PHT biosynthetic gene cluster Pseudomonas savastanoi pv. phaseolicola