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Literature summary for 2.1.3.3 extracted from

  • Yu, W.; Lin, Y.; Yao, J.; Huang, W.; Lei, Q.; Xiong, Y.; Zhao, S.; Guan, K.L.
    Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals (2009), J. Biol. Chem., 284, 13669-13675.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine ornithine carbamoyltransferase is acetylated at lysine resiudes, including K88, which is also mutated in ornithine carbamoyltransferase-deficient patients. K88 acetylation decreases the affinity for carbamoyl phosphate, and the maximum velocity, whereas the Km for ornithine is not affected Homo sapiens

Protein Variants

Protein Variants Comment Organism
K88Q less than 1% of wild-type activity Homo sapiens
K88R mutant retains substantial enzymatic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
Carbamoyl phosphate wild-type, pH 7.7, 37°C Homo sapiens
0.15
-
Carbamoyl phosphate mutant K88R, pH 7.7, 37°C Homo sapiens
0.36
-
L-ornithine wild-type, pH 7.7, 37°C Homo sapiens
0.42
-
L-ornithine mutant K88R, pH 7.7, 37°C Homo sapiens
0.55
-
L-ornithine mutant K88Q, pH 7.7, 37°C Homo sapiens
1.24
-
Carbamoyl phosphate mutant K88Q, pH 7.7, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information ornithine carbamoyltransferase is acetylated at lysine residues, including K88, which is also mutated in ornithine carbamoyltransferase-deficient patients. K88 acetylation decreases the affinity for carbamoyl phosphate, and the maximum velocity, whereas the Km for ornithine is not affected. K88 acetylation is regulated by both extracellular glucose and amino acid availabilit Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Chang liver cell
-
Homo sapiens
-
HEK-293T cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
Homo sapiens phosphate + L-citrulline
-
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