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Literature summary for 2.1.3.3 extracted from

  • De Gregorio, A.; Battistutta, R.; Arena, N.; Panzalorto, M.; Francescato, P.; Valentini, G.; Bruno, G.; Zanotti, G.
    Functional and structural characterization of ovine ornithine transcarbamoylase (2003), Org. Biomol. Chem., 1, 3178-3185.
    View publication on PubMed
Search for more literature for 2.1.3.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 Carbamoylphosphate 37°C, pH 8.6 Ovis aries
0.087
-
 Carbamoylphosphate 37°C, pH 8.2 Ovis aries
0.25
-
 Carbamoylphosphate 37°C, pH 9.0 Ovis aries
0.25
-
 L-ornithine 37°C, pH 8.6 Ovis aries
0.33
-
 L-ornithine 37°C, pH 8.2 Ovis aries
0.45
-
 L-ornithine 37°C, pH 9.0 Ovis aries

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 3 * 34000, SDS-PAGE Ovis aries
102000
-
 gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Storage Stability

Storage Stability Organism
-20°C, 25 mM sodium phosphate, 10 mM carbamoylphosphate, 25 mM norvalien, pH 7.0, stable for months Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
 Ovis aries phosphate + L-citrulline
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 34000, SDS-PAGE Ovis aries

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 41 min, 50% residual activity Ovis aries
55
-
 16 min, 50% residual activity Ovis aries
63
-
 presence of 20 mM carbamoylphosphate plus 20 mM L-norvaline, stable Ovis aries
63
-
 presence of 20 mM carbamoylphosphate, 15 min, 50% residual activity Ovis aries
63
-
 presence of 20 mM ornithine, 3 min, 50% residual activity Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 at 10 mM ornithine Ovis aries
8.6
-
 at 0.025 mM ornithine Ovis aries