Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
delta-N-(phosphonacetyl)-L-ornithine | - |
Thermus thermophilus | |
Delta-N-Phosphonoacetyl-L-ornithine | - |
Thermus thermophilus | |
phosphate | - |
Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
3 * 36000, SDs-PAGE | Thermus thermophilus |
110000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, thermodenaturation at 85°C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.33 | - |
recombinant enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Thermus thermophilus | phosphate + L-citrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 36000, SDs-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life: 18 min, 10 mM ornithine and phosphate increase half-life 5fold and 7fold respectively | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
at an ornithine concentration around the Km value, at higher concetration the pH optimum shifts to pH 7.0 | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
delta-N-(phosphonacetyl)-L-ornithine | - |
Thermus thermophilus |