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Literature summary for 2.1.3.3 extracted from
- Ornithine carbamoyltransferase from the extreme thermophile Thermus thermophilus. Analysis of the gene and characterization of the protein (1997), Eur. J. Biochem., 248, 466-474.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| delta-N-(phosphonacetyl)-L-ornithine | - |
Thermus thermophilus |  |
| Delta-N-Phosphonoacetyl-L-ornithine | - |
Thermus thermophilus | |
| phosphate | - |
Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
3 * 36000, SDs-PAGE | Thermus thermophilus |
| 110000 | - |
gel filtration | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, thermodenaturation at 85°C, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.33 | - |
recombinant enzyme | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-ornithine | - |
Thermus thermophilus | phosphate + L-citrulline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 36000, SDs-PAGE | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life: 18 min, 10 mM ornithine and phosphate increase half-life 5fold and 7fold respectively | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
at an ornithine concentration around the Km value, at higher concetration the pH optimum shifts to pH 7.0 | Thermus thermophilus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00001 | - |
delta-N-(phosphonacetyl)-L-ornithine | - |
Thermus thermophilus | |
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Release 2023.1 (January 2023)
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