General Stability | Organism |
---|---|
cetyltrimethylammonium stabilizes enzyme in crude extract against heat inactivation in presence of the detergent | Homo sapiens |
loss of activity after the second thawing | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
114000 | - |
gel filtration, glycerol density gradient centrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
233 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 10 mM potassium phosphate, pH 7.0, 1 mM 2-mercaptoethanol, 4 months, no loss of activity | Homo sapiens |
-50°C, indefinitely | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Homo sapiens | phosphate + L-citrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | SDS-PAGE under reducing conditions | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-50 | 60 | stable | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
triethanolamine buffer, 85% of maximal activity at pH 8.5, 25% of maximal activity at pH 6.8 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.5 | approx. 85% of maximal activity at pH 6.8, approx. 25% of maximal activity at pH 8.5 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.2 | 120 min, 37°C, stable | Homo sapiens |