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Literature summary for 2.1.3.3 extracted from

  • Templeton, M.D.; Sullivan, P.A.; Sheperd, M.G.
    The inhibition of ornithine transcarbamoylase from Escherichia coli W by phaseolotoxin (1984), Biochem. J., 224, 379-388.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
N-delta-(phosphosulfamyl)ornithylalanylhomoarginine trivial name phaseolotoxin, 0.1 mM, 93% inhibition Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37500
-
3 * 37500, SDS-PAGE Escherichia coli
95000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
N-delta-(phosphonoacetyl)ornithine affinity chromatography, Sephadex G-100 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2914
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, prolonged storage, no loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
Escherichia coli phosphate + L-citrulline
-
?

Subunits

Subunits Comment Organism
trimer 3 * 37500, SDS-PAGE Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
N-delta-(phosphosulfamyl)ornithylalanylhomoarginine mixed or competitive vs. carbamoyl phosphate Escherichia coli
0.0009
-
N-delta-(phosphosulfamyl)ornithylalanylhomoarginine noncompetitive vs. ornithine Escherichia coli