Literature summary for 2.1.3.2 extracted from

Lunev, S.; Bosch, S.S.; Batista, F.d.e. .A.; Wrenger, C.; Groves, M.R.
Crystal structure of truncated aspartate transcarbamoylase from Plasmodium falciparum (2016), Acta Crystallogr. Sect. F, 72, 523-533 .

Search for more literature for 2.1.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
truncated enzyme, hanging drop vapor diffusion method, using 0.2 M sodium sulfate, 5 mM MgSO4, 15% (w/v) PEG 3350 in 0.1 M bis-Tris propane pH 7.5	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
R109A/K138A	the mutant shows significantly lower activity compared to the wild type enzyme	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
carbamoyl phosphate + L-aspartate	Plasmodium falciparum	-	phosphate + N-carbamoyl-L-aspartate	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	O15804	-	-

Purification (Commentary)

Purification (Comment)	Organism
Strep-tag affinity resin column chromatography and Superdex 75 gel filtration	Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.85	-	purified mutant enzyme R109A/K138A, at pH 8.0 and 25°C	Plasmodium falciparum
11.04	-	purified truncated wild type enzyme, at pH 8.0 and 25°C	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbamoyl phosphate + L-aspartate	-	Plasmodium falciparum	phosphate + N-carbamoyl-L-aspartate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 43300, calculated from amino acid sequence	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
aspartate transcarbamoylase	-	Plasmodium falciparum
ATC	-	Plasmodium falciparum

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Release 2023.1 (January 2023)