Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.3.2 extracted from

  • Van Boxstael, S.; Cunin, R.; Khan, S.; Maes, D.
    Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi: thermostability and 1.8 A resolution crystal structure of the catalytic subunit complexed with the bisubstrate analogue N-phosphonacetyl-L-aspartate (2003), J. Mol. Biol., 326, 203-216.
    View publication on PubMed
Search for more literature for 2.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of catalytic subunit in Escherichia coli Pyrococcus abyssi

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion, 0.002 ml of enzyme solution, 7 mg/ml, in 20 mM Tris-HCl, pH 8.2, 2 mM 2-mercaptoethanol, 300 mM NaCl, is mixed with 0.002 ml reservoir solution consisting of 1.4 M citrate, pH 6.8, X-ray structure to 1.8 A resolution Pyrococcus abyssi

Inhibitors

Inhibitors Comment Organism Structure
N-(Phosphonoacetyl)-L-aspartate 0.002 mM, 50% inhibition of catalytic subunit Pyrococcus abyssi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 Carbamoyl phosphate recombinant catalytic subunit, at 37°C Pyrococcus abyssi
12.3
-
 L-aspartate recombinant catalytic subunit, at 30°C Pyrococcus abyssi
14.1
-
 L-aspartate recombinant catalytic subunit, at 45°C Pyrococcus abyssi
15.5
-
 L-aspartate recombinant catalytic subunit, at 37°C Pyrococcus abyssi
19.7
-
 L-aspartate recombinant catalytic subunit, at 55°C Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Pyrococcus abyssi
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi P77918
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 catalytic subunit, half-life: 80 min, half-life of holenzyme: 240 min Pyrococcus abyssi
98
-
 half-life of the holoenzyme is 2.5 times higher than that of the catalytic subunit Pyrococcus abyssi