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Literature summary for 2.1.3.15 extracted from

  • Silvers, M.A.; Pakhomova, S.; Neau, D.B.; Silvers, W.C.; Anzalone, N.; Taylor, C.M.; Waldrop, G.L.
    Crystal structure of carboxyltransferase from Staphylococcus aureus bound to the antibacterial agent moiramide B (2016), Biochemistry, 55, 4666-4674 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.3.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
moiramide B bound to carboxyltransferase, sitting drop vapor diffusion method, using 0.1 M MES (pH 5.0) and 1.6 M NH4SO4 Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
(3S)-beta-phenylalanine-L-valine-(4S)-methyl-2,5-pyrrolidinedione
-
 Staphylococcus aureus
(3S)-L-valine-(4S)-methyl-2,5-pyrrolidinedione
-
 Staphylococcus aureus
(3S)-methyl-2,5-pyrrolidinedione
-
 Staphylococcus aureus
moiramide B
-
 Staphylococcus aureus
succinimide
-
 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA Staphylococcus aureus
-
 [biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
 r
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA Staphylococcus aureus
-
 [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
 Staphylococcus aureus [biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
 r
[biotin carboxyl-carrier protein]-N6-carboxybiotinyl-L-lysine + acetyl-CoA
-
 Staphylococcus aureus [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine + malonyl-CoA
-
 r

Synonyms

Synonyms Comment Organism
ACC
-
 Staphylococcus aureus
acetyl-CoA carboxylase
-
 Staphylococcus aureus
carboxyltransferase component of acetyl-CoA carboxylase Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
 moiramide B at pH 8.0, temperature not specified in the publication Staphylococcus aureus