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Literature summary for 2.1.3.12 extracted from
- New aminocoumarin antibiotics formed by a combined mutational and chemoenzymatic approach utilizing the carbamoyltransferase NovN (2004), Chem. Biol., 11, 655-662.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Streptomyces lividans as an N-terminal His6 fusion protein | Streptomyces niveus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus |  |
| 0.0024 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus | |
| 0.0143 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus | |
| Mn2+ | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78500 | - |
1 * 78500, calculated from sequence | Streptomyces niveus |
| 79000 | - |
gel filtration | Streptomyces niveus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + decarbamoylnovobiocin | Streptomyces niveus | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | phosphate + novobiocin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces niveus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-terminal His6 fusion protein | Streptomyces niveus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + decarbamoylnovobiocin | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus | phosphate + novobiocin | - |
? | |
| carbamoyl phosphate + decarbamoylnovobiocin | NovN is specific for carbamoyl phosphate as substrate. With acetyl phosphate, no product formation is observed | Streptomyces niveus | phosphate + novobiocin | - |
? | |
| carbamoyl phosphate + novclobiocin 104 | 87% of novclobiocin 104 is converted to the carbamoylated derivative after overnight incubation | Streptomyces niveus | phosphate + ? | - |
? | |
| carbamoyl phosphate + novclobiocin 105 | - |
Streptomyces niveus | phosphate + novclobiocin 115 | - |
? | |
| carbamoyl phosphate + novclobiocin 107 | - |
Streptomyces niveus | phosphate + novclobiocin 117 | - |
? | |
| carbamoyl phosphate + novclobiocin 108 | - |
Streptomyces niveus | phosphate + novclobiocin 118 | - |
? | |
| carbamoyl phosphate + novclobiocin 283 | - |
Streptomyces niveus | phosphate + novclobiocin 284 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 78500, calculated from sequence | Streptomyces niveus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NovN | - |
Streptomyces niveus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces niveus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.32 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus | |
| 1.53 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus | |
| 2.4 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Streptomyces niveus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM | Streptomyces niveus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 107 | - |
novclobiocin 105 | pH 8.0, 30°C | Streptomyces niveus | |
| 695 | - |
novclobiocin 104 | pH 8.0, 30°C | Streptomyces niveus | |
| 1000 | - |
decarbamoylnovobiocin | pH 8.0, 30°C | Streptomyces niveus | |
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