Cloned (Comment) | Organism |
---|---|
1.3S subunit cloned and expressed in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and mutant 1.3S subunit, affinity chromatography on avidin(monomeric)-agarose, copurification of apo and biotinylated 1.3S forms | Propionibacterium freudenreichii subsp. shermanii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + oxaloacetate | two partial reactions | Propionibacterium freudenreichii subsp. shermanii | (S)-methylmalonyl-CoA + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | amino acid sequence of biotinyl subunit | Propionibacterium freudenreichii subsp. shermanii |
More | the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
More | 1.3 S is homogen, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | requirement | Propionibacterium freudenreichii subsp. shermanii | |
biotin | biotin containing subunit of enzyme acts as a carboxyl carrier between the CoA ester sites on the central 12S subunit of enzyme and keto acid sites on outer 5S subunit of enzyme and links the 12S and 5S subunits together to form a 26S multisubunit enzyme complex, biotin content: 28% | Propionibacterium freudenreichii subsp. shermanii |