General Stability | Organism |
---|---|
Co2+ protects | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Guanidinium chloride | Co2+ protects | Propionibacterium freudenreichii subsp. shermanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement | Propionibacterium freudenreichii subsp. shermanii | |
Co2+ | 3 types of Co2+: tightly i.e. catalytic, not so tightly, and weakly bound, above 2 mM, stabilizes quarternary structure, no exchange between catalytic and stabilizing Co2+ | Propionibacterium freudenreichii subsp. shermanii | |
Co2+ | no formation of Co-thiol bonds | Propionibacterium freudenreichii subsp. shermanii | |
Zn2+ | slightly stabilizing | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen prevents reassembly of dissociated subunits | Propionibacterium freudenreichii subsp. shermanii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | mechanism | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.6 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
dissociation and rapid loss of activity in dilute solution in alkaline 50 mM buffer (t1/2: 12 min), Co2+ protects, Zn2+ protects slightly (t1/2: 22 min) | Propionibacterium freudenreichii subsp. shermanii |
50 | - |
denaturation within 1 min, Co2+ protects, Zn2+ protects slightly | Propionibacterium freudenreichii subsp. shermanii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
26S-form stable | Propionibacterium freudenreichii subsp. shermanii |
8 | - |
dilute solution, 50 mM buffer, 25°C: enzyme dissociates into subunits and rapidly loses activity, Co2+ protects, Zn2+ protects slightly | Propionibacterium freudenreichii subsp. shermanii |
9 | - |
enzyme dissociates into 12SH, 5SE, 6SE and 6SH subunits, in the presence of Co2+: the 6SE subunits binds to 12SH subunit | Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | requirement | Propionibacterium freudenreichii subsp. shermanii |