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Literature summary for 2.1.3.1 extracted from

  • Harmon, F.R.; Goss, N.H.; Wood, H.G.
    Stabilization of the quaternary structure of transcarboxylase by cobalt (II) ions (1982), Biochemistry, 21, 2847-2852.
    View publication on PubMed

General Stability

General Stability Organism
Co2+ protects Propionibacterium freudenreichii subsp. shermanii

Inhibitors

Inhibitors Comment Organism Structure
Guanidinium chloride Co2+ protects Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement Propionibacterium freudenreichii subsp. shermanii
Co2+ 3 types of Co2+: tightly i.e. catalytic, not so tightly, and weakly bound, above 2 mM, stabilizes quarternary structure, no exchange between catalytic and stabilizing Co2+ Propionibacterium freudenreichii subsp. shermanii
Co2+ no formation of Co-thiol bonds Propionibacterium freudenreichii subsp. shermanii
Zn2+ slightly stabilizing Propionibacterium freudenreichii subsp. shermanii

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen prevents reassembly of dissociated subunits Propionibacterium freudenreichii subsp. shermanii

Reaction

Reaction Comment Organism Reaction ID
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate mechanism Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.6
-
-
Propionibacterium freudenreichii subsp. shermanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Propionibacterium freudenreichii subsp. shermanii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
dissociation and rapid loss of activity in dilute solution in alkaline 50 mM buffer (t1/2: 12 min), Co2+ protects, Zn2+ protects slightly (t1/2: 22 min) Propionibacterium freudenreichii subsp. shermanii
50
-
denaturation within 1 min, Co2+ protects, Zn2+ protects slightly Propionibacterium freudenreichii subsp. shermanii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
26S-form stable Propionibacterium freudenreichii subsp. shermanii
8
-
dilute solution, 50 mM buffer, 25°C: enzyme dissociates into subunits and rapidly loses activity, Co2+ protects, Zn2+ protects slightly Propionibacterium freudenreichii subsp. shermanii
9
-
enzyme dissociates into 12SH, 5SE, 6SE and 6SH subunits, in the presence of Co2+: the 6SE subunits binds to 12SH subunit Propionibacterium freudenreichii subsp. shermanii

Cofactor

Cofactor Comment Organism Structure
biotin requirement Propionibacterium freudenreichii subsp. shermanii