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Literature summary for 2.1.2.9 extracted from
- Biochemical characterization of pathogenic mutations in human mitochondrial methionyl-tRNA formyltransferase (2014), J. Biol. Chem., 289, 32729-32741.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A172L | site-directed mutagenesis, the mutation causes a 6fold decrease in enzyme activity compared to wild-type, the Vmax/Km of the mutant is about 4fold lower than that of the wild-type enzyme | Escherichia coli |
| A172S | site-directed mutagenesis, the mutation does not significantly affect the enzyme activity | Escherichia coli |
| A89L | site-directed mutagenesis, the Vmax/Km of the mutant is about 144fold lower than that of the wild-type enzyme | Escherichia coli |
| A89S | site-directed mutagenesis, the mutation does not significantly affect the enzyme activity | Escherichia coli |
| S125L | naturally occurring mutation, the mutant has a 653fold lower activity compared to the wild-type | Homo sapiens |
| S209L | naturally occurring mutation, the mutant has a 36fold lower activity compared to the wild-type | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics of wild-type and mutant enzymes | Escherichia coli | |
| additional information | - |
additional information | Michaelis-Menten kinetics of wild-type and mutant enzymes with human mitochondrial mt-tRNAMet and with Escherichia coli tRNA2fMet, overview | Homo sapiens | |
| 0.00051 | - |
L-methionyl-tRNAfMet | Escherichia coli tRNA2fMet, pH 7.6, 37°C, wild-type enzyme | Escherichia coli |  |
| 0.00107 | - |
L-methionyl-tRNAfMet | Escherichia coli tRNA2fMet, pH 7.6, 37°C, mutant A172L | Escherichia coli | |
| 0.00587 | - |
L-methionyl-tRNAfMet | Escherichia coli tRNA2fMet, pH 7.6, 37°C, mutant A89L | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | Escherichia coli | - |
tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | Homo sapiens | - |
tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P23882 | - |
- |
| Homo sapiens | Q96DP5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Homo sapiens | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | Escherichia coli tRNA2fMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
| 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | human mt-tRNAMet | Homo sapiens | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mitochondrial methionyl-tRNA formyltransferase | - |
Escherichia coli |
| mitochondrial methionyl-tRNA formyltransferase | - |
Homo sapiens |
| MTF | - |
Escherichia coli |
| MTF | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia coli |
| 7.6 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyltetrahydrofolate | - |
Escherichia coli | |
| 10-formyltetrahydrofolate | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mutations of conserved enzyme residues affect enzyme activity and lead to poor formylation of mitochondrial methionyl-tRNA and thereby reduced mitochondrial translation efficiency, causing Leigh syndrome | Escherichia coli |
| malfunction | mutations of conserved enzyme residues affect enzyme activity and lead to poor formylation of mitochondrial methionyl-tRNA and thereby reduced mitochondrial translation efficiency, causing Leigh syndrome | Homo sapiens |
| physiological function | N-formylation of initiator methionyl-tRNA (Met-tRNAMet) by methionyl-tRNA formyltransferase is important for translation initiation in bacteria, mitochondria, and chloroplasts | Escherichia coli |
| physiological function | N-formylation of initiator methionyl-tRNA (Met-tRNAMet) by methionyl-tRNA formyltransferase is important for translation initiation in bacteria, mitochondria, and chloroplasts | Homo sapiens |
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