Cloned (Comment) | Organism |
---|---|
cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of the formylation of various mutant methionyl-tRNAs | Saccharomyces cerevisiae | |
0.0003 | - |
E. coli L-methionyl-tRNAfMet | - |
Saccharomyces cerevisiae | |
0.0003 | - |
E. coli L-isoleucyl-tRNAfMet | - |
Saccharomyces cerevisiae | |
0.0005 | - |
E. coli L-valyl-tRNAfMet | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Saccharomyces cerevisiae | initiation of protein synthesis in mitochondria | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains CMY214, W303, YPALS, DBY2057 | - |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzyme, expressed in Escherichia coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Saccharomyces cerevisiae | tetrahydrofolate + N-formylisoleucyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O | 250fold reduced catalytic efficiency compared with methionyl-tRNAfMet | Saccharomyces cerevisiae | tetrahydrofolate + N-formylisoleucyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-isoleucyl-tRNAfMet | - |
Saccharomyces cerevisiae | tetrahydrofolate + N-formylisoleucyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates yeast mitochondrial methionyl-tRNAfMet, specific for initiator tRNAs | Saccharomyces cerevisiae | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Saccharomyces cerevisiae | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | enzyme recognizes nucleotidic features in the acceptor stem of mitochondrial initiator tRNA, recognition mechanism | Saccharomyces cerevisiae | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | initiation of protein synthesis in mitochondria | Saccharomyces cerevisiae | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Saccharomyces cerevisiae | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | 25fold reduced catalytic efficiency compared with methionyl-tRNAfMet | Saccharomyces cerevisiae | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
additional information | mutant tRNAs as poor substrates | Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters of the formylation of various mutant methionyl-tRNAs | Saccharomyces cerevisiae | |
0.0005 | - |
L-isoleucyl-tRNAfMet | - |
Saccharomyces cerevisiae | |
0.009 | - |
L-valyl-tRNAfMet | - |
Saccharomyces cerevisiae | |
0.13 | - |
E. coli L-methionyl-tRNAfMet | - |
Saccharomyces cerevisiae |