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Literature summary for 2.1.2.9 extracted from

  • Samuel, C.E.; Rabinowitz, J.C.
    Initiation of protein synthesis by folate-sufficient and folate-deficient Streptococcus faecalis R: Partial purification and properties of methionyl-transfer ribonucleic acid synthetase and methionyl-transfer ribonucleic acid formyltransferase (1974), J. Bacteriol., 118, 21-31.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information not stimulated by ATP or GTP Escherichia coli
additional information not stimulated by ATP or GTP Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by folic acid; not inhibited by pteroic acid, aminopterin, methionyl-tRNAmMet and uncharged tRNAmMet Enterococcus faecalis
additional information E. coli A19 enzyme is not inhibited by uncharged tRNAmMet and tRNAfMet from E. coli K12 or Streptococcus faecalis Escherichia coli
tetrahydrofolate competitive inhibition Enterococcus faecalis
tRNAfMet tRNAfMet from Streptococcus faecalis or E. coli K12, strong inhibition Enterococcus faecalis
tRNAfMet non-aminoacylated tRNAfMet is a competitive inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00072 0.0009 L-methionyl-tRNAfMet
-
Enterococcus faecalis
0.006 0.007 10-formyltetrahydrofolate
-
Enterococcus faecalis
0.011 0.016 10-formyltetrahydropteroyltriglutamate
-
Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-
cytoplasm
-
Enterococcus faecalis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli
Mg2+ requirement Enterococcus faecalis
Mg2+ optimal concentration: 30 mM Escherichia coli
Mg2+ optimal concentration: 30 mM Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29900
-
sucrose density gradient centrifugation Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Enterococcus faecalis involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Enterococcus faecalis R / ATCC 8043 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli A19 involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis R / ATCC 8043
-
-
-
Escherichia coli
-
-
-
Escherichia coli A19
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
100-300fold, partial purification Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
-
Escherichia coli
0.64 0.73
-
Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Enterococcus faecalis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Enterococcus faecalis R / ATCC 8043 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli A19 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli A19 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor Enterococcus faecalis tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet
-
?
additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
?
additional information no formylation of methionyl-tRNAmMet Enterococcus faecalis ?
-
?
additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Escherichia coli ?
-
?
additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Enterococcus faecalis ?
-
?
additional information no formylation of methionyl-tRNAmMet Enterococcus faecalis R / ATCC 8043 ?
-
?
additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Enterococcus faecalis R / ATCC 8043 ?
-
?
additional information no formylation of methionyl-tRNAmMet Escherichia coli A19 ?
-
?
additional information ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet Escherichia coli A19 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli
30
-
assay at Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
about Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate
-
Escherichia coli
10-formyltetrahydrofolate
-
Enterococcus faecalis
additional information no cofactors: ribonucleotide mono- or triphosphates Escherichia coli
additional information no cofactors: ribonucleotide mono- or triphosphates Enterococcus faecalis
tetrahydrofolate
-
Escherichia coli
tetrahydrofolate
-
Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00018 0.00057 tRNAfMet
-
Enterococcus faecalis
0.024
-
tetrahydrofolate
-
Enterococcus faecalis