Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not stimulated by ATP or GTP | Escherichia coli | |
additional information | not stimulated by ATP or GTP | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by folic acid; not inhibited by pteroic acid, aminopterin, methionyl-tRNAmMet and uncharged tRNAmMet | Enterococcus faecalis | |
additional information | E. coli A19 enzyme is not inhibited by uncharged tRNAmMet and tRNAfMet from E. coli K12 or Streptococcus faecalis | Escherichia coli | |
tetrahydrofolate | competitive inhibition | Enterococcus faecalis | |
tRNAfMet | tRNAfMet from Streptococcus faecalis or E. coli K12, strong inhibition | Enterococcus faecalis | |
tRNAfMet | non-aminoacylated tRNAfMet is a competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00072 | 0.0009 | L-methionyl-tRNAfMet | - |
Enterococcus faecalis | |
0.006 | 0.007 | 10-formyltetrahydrofolate | - |
Enterococcus faecalis | |
0.011 | 0.016 | 10-formyltetrahydropteroyltriglutamate | - |
Enterococcus faecalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
cytoplasm | - |
Enterococcus faecalis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Escherichia coli | |
Mg2+ | requirement | Enterococcus faecalis | |
Mg2+ | optimal concentration: 30 mM | Escherichia coli | |
Mg2+ | optimal concentration: 30 mM | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29900 | - |
sucrose density gradient centrifugation | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Enterococcus faecalis | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Enterococcus faecalis R / ATCC 8043 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli A19 | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis R / ATCC 8043 | - |
- |
- |
Escherichia coli | - |
- |
- |
Escherichia coli A19 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
100-300fold, partial purification | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
- |
Escherichia coli |
0.64 | 0.73 | - |
Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Enterococcus faecalis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor | Enterococcus faecalis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Enterococcus faecalis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Enterococcus faecalis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor | Enterococcus faecalis R / ATCC 8043 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Enterococcus faecalis R / ATCC 8043 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Enterococcus faecalis R / ATCC 8043 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specificity is restricted to methionyl-tRNAfMet | Escherichia coli A19 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli A19 | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O | 10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor | Enterococcus faecalis | tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet | - |
? | |
additional information | no formylation of methionyl-tRNAmMet | Escherichia coli | ? | - |
? | |
additional information | no formylation of methionyl-tRNAmMet | Enterococcus faecalis | ? | - |
? | |
additional information | ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet | Escherichia coli | ? | - |
? | |
additional information | ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet | Enterococcus faecalis | ? | - |
? | |
additional information | no formylation of methionyl-tRNAmMet | Enterococcus faecalis R / ATCC 8043 | ? | - |
? | |
additional information | ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet | Enterococcus faecalis R / ATCC 8043 | ? | - |
? | |
additional information | no formylation of methionyl-tRNAmMet | Escherichia coli A19 | ? | - |
? | |
additional information | ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet | Escherichia coli A19 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
about | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Escherichia coli | |
10-formyltetrahydrofolate | - |
Enterococcus faecalis | |
additional information | no cofactors: ribonucleotide mono- or triphosphates | Escherichia coli | |
additional information | no cofactors: ribonucleotide mono- or triphosphates | Enterococcus faecalis | |
tetrahydrofolate | - |
Escherichia coli | |
tetrahydrofolate | - |
Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | 0.00057 | tRNAfMet | - |
Enterococcus faecalis | |
0.024 | - |
tetrahydrofolate | - |
Enterococcus faecalis |