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Literature summary for 2.1.2.9 extracted from

  • Blanquet, S.; Dessen, P.; Kahn, D.
    Properties and specificity of methionyl-tRNAfMet formyltransferase from Escherichia coli (1984), Methods Enzymol., 106, 141-152.
    View publication on PubMed

General Stability

General Stability Organism
EDTA or 2-mercaptoethanol stabilizes Escherichia coli
high ionic strength, e.g. 150 mM KCl stabilizes and prevents adsorption to glassware Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
L-methionyl-tRNAfMet
-
Escherichia coli
0.0135
-
10-formyltetrahydrofolate
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activity is maximum in presence of 7 mM MgCl2 and 150 mM KCl Escherichia coli
Mg2+ activity is maximum in presence of 7 mM MgCl2 and 150 mM KCl Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
1 * 32000, SDS-PAGE Escherichia coli
33000
-
sedimentation equilibrium method, gel filtration, small-angle neutron scattering Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 50%, stable to Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K12 EM20031(F32)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on Sepharose-bound tRNA Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.4
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-15°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, several months, stable Escherichia coli
25°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 150 mM KCl, 2 days, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
?
additional information broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet Escherichia coli ?
-
?
additional information no formylation of methionyl-tRNAmMet Escherichia coli K12 EM20031(F32) ?
-
?
additional information broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet Escherichia coli K12 EM20031(F32) ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
L-methionyl-tRNAfMet
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli
tetrahydrofolate
-
Escherichia coli