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Literature summary for 2.1.2.8 extracted from

  • Park, S.H.; Suh, S.W.; Song, H.K.
    A cytosine modification mechanism revealed by the structure of a ternary complex of deoxycytidylate hydroxymethylase from bacteriophage T4 with its cofactor and substrate (2019), IUCrJ, 6, 206-217 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Tequatrovirus T4

Crystallization (Commentary)

Crystallization (Comment) Organism
C148S/D179N mutant in complex with dCMP and tetrahydrofolate, hanging drop vapor diffusion method, using 100 mM Tris-HCl pH 8.5 and 0.96 M sodium citrate Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
C148S/D179N active site mutant Tequatrovirus T4
D145N the mutant shows no activity Tequatrovirus T4
D179N the mutation significantly decreases the binding affinity to dCMP Tequatrovirus T4
S94A the mutant 25% residual activity compared to the wild type enzyme Tequatrovirus T4
S94N the mutant shows no activity Tequatrovirus T4
Y96F the mutant shows no activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + H2O + dCMP Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyl-dCMP
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P08773
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + H2O + dCMP
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyl-dCMP
-
?

Synonyms

Synonyms Comment Organism
DCH
-
Tequatrovirus T4
dCMP hydroxymethylase
-
Tequatrovirus T4
deoxycytidylate hydroxymethylase
-
Tequatrovirus T4

Cofactor

Cofactor Comment Organism Structure
5,10-methenyltetrahydrofolate
-
Tequatrovirus T4