Literature summary for 2.1.2.8 extracted from

Chen, C.; Gao, T.; Zhao, G.; Deng, Z.; Hu, S.; Xu, H.; He, X.
Evidence from 18O feeding studies for hydroxyl group donor in the reaction catalyzed by cytidylate hydroxymethylase MilA (2013), Chin. Sci. Bull., 58, 864-868.

No PubMed abstract available

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	Tequatrovirus	the hydroxymethylation is catalyzed by dCMP hydroxymethylase, which transfers a methylene group from methylenetetrahydrofolate to C5 of dCMP and then uses a water molecule to hydrate the methylene group to form hydroxymethyl-dCMP, one precursor for phage DNA replication	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	the hydroxymethylation is catalyzed by dCMP hydroxymethylase, which transfers a methylene group from methylenetetrahydrofolate to C5 of dCMP and then uses a water molecule to hydrate the methylene group to form hydroxymethyl-dCMP, one precursor for phage DNA replication	Tequatrovirus	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	-	?
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate	the enzyme uses the solvent water as the hydroxyl group donor	Tequatrovirus	tetrahydrofolate + 5-hydroxymethyldeoxycytidylate	-	?
additional information	5-hydroxymethylcytosine in phage is formed by hydroxymethylation of the cytosine base in deoxycytidylate (dCMP) by deoxycytidylate hydroxymethylase, which uses the solvent water as the hydroxyl group donor	Tequatrovirus	?	-	?

Synonyms

Synonyms	Comment	Organism
dCMP hydroxymethylase	-	Tequatrovirus
deoxycytidylate hydroxymethylase	-	Tequatrovirus

Cofactor

Cofactor	Comment	Organism	Structure
5,10-methylenetetrahydrofolate	-	Tequatrovirus

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Release 2023.1 (January 2023)