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Literature summary for 2.1.2.8 extracted from
- Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene (1988), Eur. J. Biochem., 172, 553-563.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and overexpression of g42 in Escherichia coli K38 | Tequatrovirus T4 |
| nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids | Escherichia phage T2 |
| nucleotide sequence of dCMP hydroxymethylase gene encoding a protein of 246 amino acids | Enterobacteria phage T6 |
| nucleotide sequence of g42, the dCMP hydroxymethylase gene, encodes a protein of 246 amino acids | Tequatrovirus T4 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transition mutations in gene 42 | Tequatrovirus T4 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
2 * 27000, SDS-PAGE | Tequatrovirus T4 |
| 28450 | - |
calculation from the amino acid sequence | Tequatrovirus T4 |
| 28450 | - |
calculation from the amino acid sequence | Escherichia phage T2 |
| 28450 | - |
calculation from the amino acid sequence | Enterobacteria phage T6 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacteria phage T6 | - |
- |
- |
| Escherichia phage T2 | - |
- |
- |
| Tequatrovirus T4 | - |
BK 536 and JSW 800, grown on Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tequatrovirus T4 |
- |
Enterobacteria phage T6 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.865 | - |
- |
Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? |  |
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Escherichia phage T2 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
| 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Enterobacteria phage T6 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 27000, SDS-PAGE | Tequatrovirus T4 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 333 mol substrate per min per 56.8 kg protein | Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methylenetetrahydrofolate | - |
Tequatrovirus T4 | |
| 5,10-methylenetetrahydrofolate | - |
Escherichia phage T2 | |
| 5,10-methylenetetrahydrofolate | - |
Enterobacteria phage T6 | |
| tetrahydrofolate | - |
Tequatrovirus T4 | |
| tetrahydrofolate | - |
Escherichia phage T2 | |
| tetrahydrofolate | - |
Enterobacteria phage T6 | |
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