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Literature summary for 2.1.2.7 extracted from
- Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme (2008), Biosci. Biotechnol. Biochem., 72, 3002-3005.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aminobacter sp. AJ110403 |
| expression in Escherichia coli | Ensifer sp. AJ110404 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
tetrahydrofolate | - |
Aminobacter sp. AJ110403 |  |
| 0.228 | - |
tetrahydrofolate | - |
Ensifer sp. AJ110404 | |
| 1.5 | - |
2-methyl-L-serine | - |
Aminobacter sp. AJ110403 | |
| 1.88 | - |
2-methyl-L-serine | - |
Ensifer sp. AJ110404 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
gel filtration | Aminobacter sp. AJ110403 |
| 80000 | - |
gel filtration | Ensifer sp. AJ110404 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aminobacter sp. AJ110403 | B2DEU8 | - |
- |
| Ensifer sp. AJ110404 | B2DEV1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| column chromatography, 16.4% yield | Ensifer sp. AJ110404 |
| column chromatography, 72.8% yield | Aminobacter sp. AJ110403 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.25 U/mg, cell-free extract | Ensifer sp. AJ110404 |
| additional information | - |
4.75 U/mg, cell-free extract | Aminobacter sp. AJ110403 |
| additional information | - |
7.10 U/mg, after purification | Ensifer sp. AJ110404 |
| additional information | - |
8.32 U/mg, after purification | Aminobacter sp. AJ110403 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methyl-L-serine + tetrahydrofolate | assay at pH 7.4, 30°C | Aminobacter sp. AJ110403 | D-alanine + 5,10-methylenetetrahydrofolate | - |
r | |
| 2-methyl-L-serine + tetrahydrofolate | assay at pH 7.4, 30°C | Ensifer sp. AJ110404 | D-alanine + 5,10-methylenetetrahydrofolate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Aminobacter sp. AJ110403 |
| homodimer | - |
Ensifer sp. AJ110404 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-methylserine hydroxymethyltransferase | - |
Aminobacter sp. AJ110403 |
| alpha-methylserine hydroxymethyltransferase | - |
Ensifer sp. AJ110404 |
| MSHMT | - |
Aminobacter sp. AJ110403 |
| MSHMT | - |
Ensifer sp. AJ110404 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aminobacter sp. AJ110403 |
| 30 | - |
assay at | Ensifer sp. AJ110404 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
value about | Aminobacter sp. AJ110403 |
| 50 | - |
value about | Ensifer sp. AJ110404 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Aminobacter sp. AJ110403 |
| 7.4 | - |
assay at | Ensifer sp. AJ110404 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Aminobacter sp. AJ110403 |
| 6 | 7.5 | - |
Ensifer sp. AJ110404 |
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