Cloned (Comment) | Organism |
---|---|
cloning and sequencing of the cDNA coding for p60, the chicken homologue of bifunctional formiminotransferase cyclodeaminase, a protein of 541 amino acids | Gallus gallus |
expressed in HeLa cells | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gallus gallus | 5829 | - |
Golgi membrane | localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures | Gallus gallus | 139 | - |
Golgi vesicle | localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures | Gallus gallus | - |
- |
microtubule | microtubule-binding protein | Gallus gallus | 5874 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59100 | - |
x * 59100, calculated from the amino acid sequence | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formiminoglutamate + tetrahydrofolate | Gallus gallus | in addition to its enzymatic activity enzyme may have a second physiological function in mediating interaction of Golgi-derived membranes with microtubules | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
N-formiminoglutamate + tetrahydrofolate | Gallus gallus | histidine degradation | 5-formiminotetrahydrofolate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | Q9YH58 | chicken, several isoforms of p60, bifunctional formiminotransferase cyclodeaminase | - |
Meleagris gallopavo | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | p60, bifunctional formiminotransferase cyclodeaminase, is post-translationally modified | Gallus gallus |
Purification (Comment) | Organism |
---|---|
purification from liver | Meleagris gallopavo |
purification from liver | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma cell | DU249 chicken hepatoma cells, contain p60 but no measurable formiminotransferase activity, may be due to the low expression of p60 | Gallus gallus | - |
liver | - |
Meleagris gallopavo | - |
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | p60: bifunctional formiminotransferase cyclodeaminase, can mediate interaction between trans-Golgi network derived vesicles and microtubule in vitro, p60 may bind to the polyglutamate on microtubules, globular hydrophilic protein | Meleagris gallopavo | ? | - |
? | |
additional information | p60: bifunctional formiminotransferase cyclodeaminase, can mediate interaction between trans-Golgi network derived vesicles and microtubule in vitro, p60 may bind to the polyglutamate on microtubules, globular hydrophilic protein | Gallus gallus | ? | - |
? | |
N-formiminoglutamate + tetrahydrofolate | in addition to its enzymatic activity enzyme may have a second physiological function in mediating interaction of Golgi-derived membranes with microtubules | Gallus gallus | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
N-formiminoglutamate + tetrahydrofolate | histidine degradation | Gallus gallus | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
tetrahydrofolate + N-formimino-L-glutamate | - |
Meleagris gallopavo | 5-formiminotetrahydrofolate + L-glutamate | - |
? | |
tetrahydrofolate + N-formimino-L-glutamate | - |
Gallus gallus | 5-formiminotetrahydrofolate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59100, calculated from the amino acid sequence | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.13 | Gallus gallus |