Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
expressed in Escherichia coli Rosetta (DE3) pLysS cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0346 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli | |
0.111 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.245 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Escherichia coli | - |
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P15639 | - |
- |
Escherichia coli K12 | P15639 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Ni-NTA column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli K12 | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli K12 | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
5-aminoimidazole-4-carboxamide ribonucleotide tranformylase | - |
Escherichia coli |
5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
AICAR tranformylase | - |
Escherichia coli |
AICAR tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
PurH | - |
Escherichia coli |
PurH | bifunctional enzyme with tranformylase and IMP cyclohydrolase activities | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.63 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli | |
4.97 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
7.18 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions | Escherichia coli |
metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.3 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
44.77 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
4150 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli |