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Literature summary for 2.1.2.3 extracted from

  • Sah, S.; Shah, R.; Govindan, A.; Varada, R.; Rex, K.; Varshney, U.
    Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in Escherichia coli (2018), Microbiology, 164, 982-991 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli
expressed in Escherichia coli Rosetta (DE3) pLysS cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0346
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli
0.111
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
0.245
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide Escherichia coli
-
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P15639
-
-
Escherichia coli K12 P15639
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli K12 dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli K12 tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
5-aminoimidazole-4-carboxamide ribonucleotide tranformylase
-
Escherichia coli
5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase
-
Escherichia coli
AICAR tranformylase
-
Escherichia coli
AICAR tranformylase/IMP cyclohydrolase
-
Escherichia coli
PurH
-
Escherichia coli
PurH bifunctional enzyme with tranformylase and IMP cyclohydrolase activities Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.63
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli
4.97
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
7.18
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

General Information

General Information Comment Organism
malfunction growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions Escherichia coli
metabolism the enzyme is involved in the folate recycling pathway Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29.3
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
44.77
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
4150
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli