Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | methotrexate, MTX, cannot directly inhibit AICAR transformylase, but blocks the AICAR transformylase process in patients with rheumatoid arthritis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 10-formyl-7,8-dihydrofolate has an about 5fold kinetic advantage, Vm/Km, over 10-formyl-5,6,7,8-tetrahydrofolate for AICAR transformylases in human leukemia cells and for human recombinant AICAR transformylase | Homo sapiens | |
additional information | - |
additional information | 10-formyl-7,8-dihydrofolate has an about 5fold kinetic advantage, Vm/Km, over 10-formyl-5,6,7,8-tetrahydrofolate for AICAR transformylases in rat bone marrow cells | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | AICAR transformylase does not form clusters | Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Homo sapiens | 10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Rattus norvegicus | 10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
additional information | Homo sapiens | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate | ? | - |
? | |
additional information | Rattus norvegicus | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow cell | - |
Rattus norvegicus | - |
carcinoma cell | - |
Homo sapiens | - |
leukemia cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | 10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase | Homo sapiens | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | 10-formyl-7,8-dihydrofolate is the in vivo substrate for AICAR transformylase | Rattus norvegicus | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | 10-formyl-7,8-dihydrofolate is kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylas | Homo sapiens | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyl-7,8-dihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | 10-formyl-7,8-dihydrofolate is kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylas | Rattus norvegicus | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Homo sapiens | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Rattus norvegicus | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
r | |
additional information | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate | Homo sapiens | ? | - |
? | |
additional information | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase. Bioactivity of the unnatural isomer [6R]-5-formyltetrahydrofolate is in vivo converted to 10-formyl-7,8-dihydrofolate and serves thus as a substrate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AICAR transformylase | - |
Homo sapiens |
AICAR transformylase | - |
Rattus norvegicus |
aminoimidazolecarboxamide ribotide transformylase | - |
Homo sapiens |
aminoimidazolecarboxamide ribotide transformylase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N10-formyl-7,8-dihydrofolate | kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylase | Homo sapiens | |
N10-formyl-7,8-dihydrofolate | kinetically preferred over 10-formyl-5,6,7,8-tetrahydrofolate by AICAR transformylase | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | aminoimidazolecarboxamide ribotide transformylase is involved in the de novo purine nucleotide biosynthesis | Rattus norvegicus |
metabolism | aminoimidazolecarboxamide ribotide transformylase is involved in the de novo purine nucleotide biosynthesis. No enzyme complex that generates 10-formyl-5,6,7,8-tetrahydrofolate and immediately channels or furnishes it to AICAR transformylase is needed because the first oxidation product of 10-formyl-5,6,7,8-tetrahydrofolate is 10-formyl-7,8-dihydrofolate that is utilized by this transformylase | Homo sapiens |
additional information | structure and active site of AICAR transformylase are not consistent with other enzymes that utilize 10-formyl-5,6,7,8-tetrahydrofolate. Methotrexate blockage of the AICAR transformylase process in patients with rheumatoid arthritis suggests that dihydrofolate reductase is involved and is consistent with dihydrofolate and 10-formyl-7,8-dihydrofolate being the product and substrate for AICAR transformylase | Homo sapiens |
additional information | structure and active site of AICAR transformylase are not consistent with other enzymes that utilize 10-formyl-5,6,7,8-tetrahydrofolate. Methotrexate blockage of the AICAR transformylase process in patients with rheumatoid arthritis suggests that dihydrofolate reductase is involved and is consistent with dihydrofolate and 10-formyl-7,8-dihydrofolate being the product and substrate for AICAR transformylase | Rattus norvegicus |
physiological function | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase, an enzyme in purine nucleotide biosynthesis de novo, which introduces C2 into the purine ring | Homo sapiens |
physiological function | 10-formyl-7,8-dihydrofolate, not 10-formyl-5,6,7,8-tetrahydrofolate, is the predominant in vivo substrate for mammalian aminoimidazolecarboxamide ribotide transformylase, an enzyme in purine nucleotide biosynthesis de novo, which introduces C2 into the purine ring | Rattus norvegicus |