Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography, and gel filtration | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
PurN | - |
Streptococcus mutans |