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Literature summary for 2.1.2.2 extracted from
- On the structural and functional modularity of glycinamide ribonucleotide formyltransferases (2003), Protein Sci., 12, 2206-2214.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202), GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202),GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.99 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens |  |
| 0.99 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens | |
| 4 | - |
beta-glycinamide ribonucleotide | wild-type enzyme | Homo sapiens | |
| 4 | - |
10-formyl-5,8-dideazafolate | wild-type enzyme | Homo sapiens | |
| 4 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) | Homo sapiens | |
| 4 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) | Homo sapiens | |
| 12 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) | Homo sapiens | |
| 12 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) | Homo sapiens | |
| 21 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) | Homo sapiens | |
| 21 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) | Homo sapiens | |
| 33 | - |
beta-glycinamide ribonucleotide | chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) | Homo sapiens | |
| 33 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyl-5,8-dideazafolate + beta-glycinamide ribonucleotide | - |
Homo sapiens | 5,8-dideazafolate + N-formyl beta-glycinamide ribonucleotide | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) | Homo sapiens | |
| 0.0034 | - |
beta-glycinamide ribonucleotide | wild-type enzyme | Homo sapiens | |
| 0.0059 | - |
10-formyl-5,8-dideazafolate | wild-type enzyme | Homo sapiens | |
| 0.018 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) | Homo sapiens | |
| 0.0262 | - |
beta-glycinamide ribonucleotide | chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) | Homo sapiens | |
| 0.031 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) | Homo sapiens | |
| 0.043 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens | |
| 0.0662 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) | Homo sapiens | |
| 0.102 | - |
10-formyl-5,8-dideazafolate | chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) | Homo sapiens | |
| 0.262 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) | Homo sapiens | |
| 0.293 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) | Homo sapiens | |
| 0.63 | - |
beta-glycinamide ribonucleotide | chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) | Homo sapiens | |
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