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Literature summary for 2.1.2.2 extracted from
- Tight binding of folate substrates and inhibitors to recombinant mouse glycinamide ribonucleotide formyltransferase (1997), Biochemistry, 36, 10506-10516.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Escherichia coli |
| medicine | - |
Homo sapiens |
| medicine | inhibitor 5,10-dideazatetrahydrofolate is antiproliferate and cytotoxic against mammalian tumor cells in culture and have broad spectrum activity against transplantable mouse tumors and human tumor xenografts | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA, recombinant enzyme expressed in insect cells with a recombinant baculovirus | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 10-formyl-5,8-dideazafolic acid hexaglutamate | - |
Mus musculus |  |
| 2',5'-thiophene dideazatetrahydrofolate | LY309887, diastereomer B | Homo sapiens | |
| 2',5'-thiophene dideazatetrahydrofolate | LY309887, diastereomer B | Mus musculus | |
| 2-deaminodideazatetrahydrofolate | - |
Mus musculus | |
| 5,10-dideazatetrahydrofolate | - |
Escherichia coli | |
| 5,10-dideazatetrahydrofolate | - |
Homo sapiens | |
| 5,10-dideazatetrahydrofolate | - |
Mus musculus | |
| 5,8-Dideazafolate | - |
Escherichia coli | |
| 5,8-dideazafolate hexaglutamate | - |
Mus musculus | |
| 5,8-dideazapteroyl hexaglutamate | - |
Mus musculus | |
| 5-deazatetrahydrofolate | - |
Mus musculus | |
| dideazatetrahydrofolate | - |
Mus musculus | |
| dideazatetrahydrofolate hexaglutamate | - |
Mus musculus | |
| homo-dideazatetrahydrofolate | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000075 | - |
10-formyl-5,8-dideazafolate | recombinant enzyme | Mus musculus | |
| 0.0013 | - |
10-formyl-5,8-dideazafolate | - |
Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Gallus gallus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Mus musculus | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Escherichia coli | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Homo sapiens | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | Mus musculus | carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
human | - |
| Mus musculus | - |
mouse | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| L-1210 cell | murine leukemia | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Mus musculus | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
| 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Escherichia coli | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
| 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide | - |
Homo sapiens | 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | - |
Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Gallus gallus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Escherichia coli | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway | Homo sapiens | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide | carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway | Mus musculus | tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide | - |
? | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase | Gallus gallus | ? | - |
? | |
| additional information | trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase | Mus musculus | ? | - |
? | |
| additional information | trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GARFT | - |
Gallus gallus |
| GARFT | - |
Mus musculus |
| GARFT | - |
Escherichia coli |
| GARFT | - |
Homo sapiens |
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